Effects Of Ginkgo Btloba Extract On Expressions Of IL1, IL-6and TNF-a In The Brain Tissues Of The Rats With Severe Acute Pancreatitis

Objective:To establish a brain injury model for Winstar rats with severe acute pancreatitisand correspondingly investigate the effects of Ginkgo biloba extract on expression ofIL–1,IL-6and TNF-a in the brain tissues of the rats with severe acute pancreatitis.And furtherto explore the mechanism of severe acute pancreatitis and the curing effects of Ginkgo lLeafextract on brain injury,and eventually provide a new idea to treat pancreaticncephalopathy.Methods:Winstar rats were chosen as experimental animals,which weredivided into normal contrl group,model group and treatment group.For rats in the controlgroup,only conversion of pancreas was performed by addomen opening,followed by woundclosure immediately.For rats in the model group and treatment group,5%Sodiumtaurocholate hydrate（0.2ml/100g）were injected under pancreatic capsule to establish braininjury model for rats with severe acute pancreatitis.For the treatment and model group,Ginkgobiloba extract (Ginaton) and normal saline(1ml/100g）were infected into intra-abdomen beforethe wound closure and injected repeatedly every8hours.The changes of following indexeswere observed after6,12and24hours after the model established,which included the quantityof serum amylase,pathogenic changes of pancreas tissues(Gross morphology observation andlight microscope method),pathogenic changes of brain tissues（light microscope and electronmicroscope method),the expressions of IL-1,IL-6and TNF-α（immunohistochemistry method)in the pancreas and brain tissues,as well as the permeability of blood brain barrier（lanthanumparticles infiltration method).Results：1.For the model group,the serum amylase showedobviously increase at early6hours after the model was established and reached the peak at24hours,which was significantly different from that of the control group（P<0.01）. When tocompare this index between the treatment group and the model group,the difference wassignificant（P<0.01）.2.For the model group,the expressions of IL-1,IL-6and TNF-α wereincreased in both pancreas and brain tissues.Also,their expressions were increased with the extention of time and were significantly different comparing with those of the normal group atthe same experimental periods(P<0.01).Moreover,the expressions of above three factors inpancreas and brain tissues were positively correlated with each other.At the sameexperimental periods,the expressions of above three factors in the treatment group were allsignificantly decreased,comparing with those in the normal group(P<0.01).3.After the modelwas established,pathogenic injury scores of the brain tissues were increased with the extentionof time and pathogenic changes were aggravated meanwhile,which were positively corrlatedwith the expressions of IL-1,IL-6,TNF-α,as well as the degrees of serum amylase.4. Theresults of electron microscope showed that for the treatment group of rats after24hours,thepermeability of blood brain barrier to lanthanum particles was lower than that of the modelgroup.And the degree of injury to brain cells was lowered in the former group.Conclusions：1. Severe acute pancreatitis can lead to brain injury at different degrees.2.For the rats with severe acute pancreatitis, the expressions of IL-1,IL-6and TNF-α inpancreas and brain tissues were significantly increased,and showed a positive correlation withthe pathogenic injury degree of pancreas and brain tissues.3.Ginkgo biloba extract cansuppress the expressions of IL-1,IL-6and TNF-α in pancreas and brain tissues of theexperimental rats.Moreover, of Ginkgo biloba extract can improve the permeability of bloodbrain barrier and showed protective effects to the brain issues.4. Ginkgo biloba extractshowed curing effects on brain tissues of the rats with severe acute pancreatitis.