Investigation Into The Relationship Between The Expression Of LIN28A And PLK4 In Epithelial Ovarian Cancer

Objective: To investigate the relationship between the expression of LIN28 A and PLK4 in epithelial ovarian cancer and provide a foundation for the further study of the mechaniam of LIN28 A and PLK4 in ovarian cancer.Methods:(1) The m RNA levels of LIN28 A and PLK4 were detected by q-PCR in different epithelial ovarian cancer cell lines and normal epithelial ovarian cell line. The expression of LIN28 A and PLK4 protein was detected by western blot in these cell lines.(2) Cell proliferation of 293 T cell lines transfected with Lin28 A, PLK4 or Lin28 A plus PLK4 vectors respectively was evaluated and the expression of LIN28 A and PLK4 in these cell lines was detected with q-PCR and western blot.(3) 79 specimens of patients were obtained from the pathology department of the affiliated hospital of Jiangsu university and the affiliated people’s hospital of Jiangsu university between 2009 and 2014 and were divided into two groups:specimens with benign epithelial ovarian tumor and specimens with ovarian epithelial cancer. Clinic data of patients were collected and post-operation follow-up was carried out till July 2015. Every specimen of both groups was handled with pathological section. The m RNA levels of LIN28 A and PLK4 were detected by q-PCR in ovarian tissues of patients. The expression of LIN28 A and PLK4 protein was detected by immunohistochemistry(IHC) in ovarian tissues. Statistical analysis was performed to analyze the relationship between LIN28 A and PLK4 protein expression and clinical characteristics of epithelial ovarian cancer and prognosis of patients with epithelial ovarian cancer.Results:(1) The m RNA levels of LIN28 A were higher in A2780 and HO8910 epithelial ovarian cancer cell lines than in normal epithelial ovarian cell line Hosepic. The m RNA levels of PLK4 were higher in A2780, SKOV3 and HO8910 epithelial ovarian cancer cell lines than in Hosepic.(2) The expression levels of LIN28 A were higher in 293 T transfected with Lin28 A,PLK4 or Lin28 a plus PLK4 vectors respectively than in the control group. Whilethe expression levels of PLK4 were higher in 293 T transfected with PLK4 or Lin28 a plus PLK4 vectors respectively than in the control group. The cell proliferation of 293 cells transfected with PLK4 or Lin28 a plus PLK4 vectors respectively was faster than the control group, while trasfected with Lin28 A did not change the proliferation of cells.(3) The m RNA levels of LIN28 A and PLK4 in epithelial ovarian cancer were higher than in benign epithelial ovarian tumor. LIN28 A and PLK4 expression in epithelial ovarian cancer was higher than in benign epithelial ovarian tumor(P<0.05). The expression of LIN28 A and PLK4 was implicated in advanced pathological stage(P<0.05), and PLK4 expression was positively correlated to LIN28A(r = 0.555, P = 0.039). As analyzed by Logistic regression analysis,LIN28 A, PLK4 and pathological stage were risk factors of prognosis of ovarian epithelial cancer patients. The survival time of ovarian epithelial cancer patients with LIN28 A and PLK4 positive expression was shorter than other patients showed by Kaplan-Meier analysis(P<0.05).Conclusion: LIN28 A and PLK4 may be associated with the poor prognosis of ovarian epithelial cancer and may be potential biomarkers of prognosis with ovarian epithelial cancer and therapeutic targets.