Study On The Relationship Between Zinc-alpha 2 Glycoprotein And Insulin Resistance

Objective:The purpose of this project is to study the role of zinc-α2 glycoprotein(ZAG) in insulin resistance(IR) and the relation between ZAG and serum insulin and insulin resistance index.We also study the relation between ZAG and adipokines such as adiponectin(ADP), leptin(LEP), tumor necrosis factor alpha(TNF-α) and interleukin IL- 6(IL-6) expression. To make a further understanding of the relationship between serum ZAG and IR.Methods:Collected 30 patients who were newly diagnosed with type 2 diabetes mellitus(T2DM) in Shanxi Province People’s Hospital endocrinology in June 2014 to 2015 March.Their fasting blood glucose was measured with 75 g OGTT.Meanwhile collecting 30 patients who were overweight in the hospital physical examination center and30 normal persons, laboratory determination of fasting blood glucose(FBG),fasting insulin calculate insulin resistance index(HOMA-IR). Enzyme linked immuno sorbent assay(ELISA) to detect ZAG, ADP, LEP, TNF-a and IL-6 levels, using SPSS17.0 statistical analysis; data inspection, in line with the normality test Test,variance homogeneity of variance analysis, do not conform to the row rank sum test,P < 0.05 indicated that the difference was statistically significant; the correlation between the use of Pearson correlation analysis and Logistic multivariate stepwise regression analysis.Results:1.The serum ZAG and fasting blood glucose were negative correlation(r=-0.634,P < of 0.05), diabetes group and overweight serum ZAG was significantly lower than that of the normal blood glucose group(P < 0.05); The serum ZAG in the T2 DM group and overweight had no significant differences(P = 0.19 > 0.05);2.serum ZAG level was negatively correlated with serum insulin(r=-0.737, P <0.05), which may be related to the early stage of insulin level in obese and type 2diabetes. Serum ZAG level was negatively correlated with HOMA-IR(r=-0.928, P <0.05);3.the insulin resistance index in the overweight and obesity group was significantly higher than that in the normal group(P<0.05), and there was no significant difference in the insulin resistance index(P=0.822>0.05) between the diabetic group and the overweight group;4.the level of serum ZAG was negatively correlated with BMI(r=-0.730, P <0.05);5.the level of serum ZAG was positively correlated with the level of serum ADP(r=0.948, P < 0.05);6.the level of serum ZAG was negatively correlated with the level of serum LEP(r=-0.782, P < 0.05);7.the level of serum ZAG was negatively correlated with the level of TNF- in serum(r=-0.950, P < 0.05);8.the level of serum ZAG was negatively correlated with the level of IL-6(r=-0.869, P < 0.05);Conclusion:The serum ZAG was positive correlation with ADP,and negatively correlated with the expression of TNF-α, LEP and IL-6, which may play a role in reducing insulin resistance, which can be used as a target for the prevention and early treatment of insulin resistance.