| Objective: To investgate the relationship of CD36 gene single nucleotide polymorphism(SNP) rs3173798, rs7755, rs1049673, rs3211956 and some of the active site of CD36 genes of g:80661113, g:80674385-80674400 between premature coronary heart disease(CHD). Methods: The subjects of the study screened from the persons who underwent coronary angiography since January 2009- January 2015, according to the coronary artery lesions, the object were divided into disease group(premature CHD) and the control group(coronary normal group). Premature CHD was defined as men’s age < 55 years, and female’s < 65 years old.Coronary normal group was defined as: the age should be same with group of disease, or male > 55 years, women > 65 years old. For all selected subjects, are asking and record the name, age, gender, hospital number and length of hospital stay, and record whether there is history of high blood pressure, diabetes, stroke, smoking and drinking. Fasting peripheral venous blood was sampled to test: 1. The detection of triglyceride, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, low density lipoprotein cholesterol, fasting blood sugar. 2. The separation of white blood cells within 30 minutes after 3 ml of anticoagulant,- 80 ℃ refrigerator for inspection, used to extract genomic DNA. Results: 1. Compared with control group, the ratio of male patients, patients with diabetes and patients with a history of smoking ratio is higher in the premature CHD group(p< 0.05). The levels of TC, LDL- C is significantly higher than control group(p< 0.05). The levels of serum creatinine were higer in the premature CHD group than those in controls(p< 0.05). Patients the premature CHD group had lower left ventricular ejection fraction measured by echocardiogragh than the controls. 2. CD36 gene SNP rs1049673 three genotype frequencies is different in the two groups, the difference was statistically significant(p < 0.05). In rs1049673 of the comparison of three different genotypes of patients with diabetes and sex and serum LDL, there are differences in the levels of TC, and the difference was statistically significant(p < 0.05). 3. CD36 gene SNP(rs3173798) have C and T two alleles, including C/T, C/C, T/T three genes. Three genotype frequency and the alleles C, T was no difference in the two groups(p > 0.05). In rs3173798 of the comparison of three different genotypes of patients,male, HBP, DM, stroke, smoking history, and lipid levels(TC, TG, LDL, HDL- C- C) were no statistical significance(p > 0.05). 4. CD36 gene SNP rs7755 three genotype frequencies is different in the two groups, the difference was statistically significant(p < 0.05). In rs7755 of the comparison of three different genotypes of patients, there are differences in the levels of LDL, and the difference was statistically significant(p < 0.05).5.CD36 gene SNP rs3211956 three genotype frequencies is different in the two groups, the difference was statistically significant(p < 0.05). In rs3211956 of the comparison of three different genotypes of patients with diabetes and sex and serum LDL, there are differences in the levels of TC, but the difference was not statistically significant(p >0.05).6. Deficiency mutation at g:80661113 in CD36 gene exon 5 was found at a frequency of 1.5% in all subjects, and the frequency was significantly higher in WT group. The two genotypes of g:80661113, the proportion of people with diabetes have difference, del CA group is significantly higher than not missing, the difference was statistically significant(p < 0.01). In addition, the plasma of the levels of TC, LDL, HDL also has difference, low del CA group, the difference was statistically significant(p< 0.05). 7. CD36 gene g:80674385-80674400 sites differ in the two groups, the difference was not statistically significant(p>0.05). The clinical characteristics of the two genotypes g:80674385-80674400 compare age, diabetes, high blood pressure, the proportion of stroke patients and serum LDL, TC level difference was not statistically significant(p> 0.05). 8. Risk factors of PCHD were analyzed by multi-factor logistic regression, the results shown in LDL levels and early onset coronary artery disease in diabetes history is related factors of the disease, and HDL levels is protective factors. Conclusion: This study shows that the CD36 rs3173798 polymorphism, rs7755, rs1049673, rs3211956 associated with the occurrence of premature coronary artery disease, in addition, the activity of CD36 g:80661113, g:80674385-80674400 is also associated with the onset of premature CHD. |
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