Yangjing Capsule Impact VEGF、VEGFR And NO Of Leydig Cell

Objective:To observe the effects of Yangjing capsule extract on VEGF and its receptor and NO in MLTC-lcells.Methods:In the petri dish after cell culture to logarithmic phase,collection of cells,use of trypsin digestion,adjust the cell concentration of 4×105/mL, divided into blank, low, medium and high dose group (0 mg/mL,0.01 mg/mL,0.1 mg/mL,1 mg/mL) vaccination within 6 orifice, set to 3 after hole, cell abundance reaches 50%, in exchange for nutrient solution containing better capsule extract cells continue to develop.24 hours after collecting cells and extract mRNA or protein, respectively by rt-pcr and Western Blot to analyze the expression of VEGF and its receptors and NO.Results:Yangjing capsule extract on MLTC-1 cells after 24 h,found VEGF and FLK on mRNA level no statistical differences between the low dose group and blank group,and high dose group and middle dose group compared with the blank group, significant difference;In protein level, low, medium and high dose group were significantly difference compared with the blank group.Yangjing capsule extract on MLTC-1 cells after 24 h,Found eNOS on mRNA level no statistical differences between the low dose group and blank group,And high dose group and middle dose group compared with the blank group, significant difference;In protein level, low, medium and high dose group were significantly difference compared with the blank group.Conclusion:Yangjing capsule extract can improve mRNA and protein expression of the VEGF and its receptors and NO n MLTC-1 cells, and this change will increase with the increase of the dose. With the increase of VEGF and its receptors, NO expression, will be on the improvement of the testis microcirculation play a role, so as to promote the formation of microvascular, provide a better environment for the beginning of sperm...