The Influences Of Flos Puerariae On Alcoholic Liver Disease And Its Toxicity

Objective:To establish an experimental model of alcoholic liver injury in rats. A research platform was build on alcoholic liver injury.To investigate the effects of Flos Puerariae on alcoholic liver injury by ethanol in rats and discuss the mechanism. Toxicity were tested to observe toxicological situation and evaluate the safety. The research aimed to provide experimental basis for its comprehensive utilization.Methods:1. (1) To establish an experimental model of alcoholic liver injury in rats. SD rats were randomly divided into 2 groups:normal group, model group. Mice in the normal group were treated with distilled water and model group were treated with 50% ethanol(once per day) Samples were taken at the end of 4th week. The activities of alanine aminotransferase (ALT),aspartate aminotransferase (AST),the contents of triglycerides(TG),dismutase (SOD),maleic dialdehyde (MDA),GSH and protein carbonyl in serum were measured.The activities of dismutase (SOD),maleic dialdehyde (MDA),GSH and protein carbonyl in liver tissue were measured.An extra sample of liver were stained with hematoxylin-eosin(HE) and examined under light microscope for general histopathology examination. (2) After model of alcoholic liver injury in rats were establisged. SD rats were randomly divided into 7 groups: normal group, model group, low,middle, high dosage of Flos Puerariae groups((0.675g/kg,1.25g/kg,2.5g/kg,5.0g/kg)) and positive drug group. Mice in the normal group were treated with distilled water and other groups were treated with 50% ethanol in the morning. Mice in the Flos Puerariae groups were treated with Flos Puerariae extract and positive drug group were treated with silymarin in the afternoon. Samples were taken at the end of 4th week. The activities of alanine aminotransferase (ALT),aspartate aminotransferase (AST),the contents of triglycerides(TG),dismutase (SOD),maleic dialdehyde (MDA),GSH and protein carbonyl in serum were measured.The activities of dismutase (SOD),maleic dialdehyde (MDA),GSH and protein carbonyl in liver tissue were measured.An extra sample of liver were stained with hematoxylin-eosin(HE) and examined under light microscope for general histopatho logy examination.2. According to 《Technical Standards for Testing & Assessment of Health Food》 Toxicologic study of Flos Puerariae include mice acute toxicity by oral administration test,gentictoxicity(Ames test,mice bone marrow polychromatic erythrocyte micronucleus test,mice sperm abnormolity test in mice) and ninety days feeding test were done.Results:1.(1) The activities of ALT, AST,MDA and protein carbonyl of the model group were significant increased compared with the normal group(P<0.05). The activities of SOD of the model group were significant increased compared with the normal group. There were serious hepatic steatosis and inflammation in the model group(P<0.05).(2)Compared with the model group, Flos Puerariae can significantly decrease ALT,AST activity and MDA,protein carbonyl content in the serum or liver tissue; Flos Puerariae can significantly increased SOD activity in the serum or liver tissue. Flos Puerariae can obviously alleviate hepatic steatosis and inflammation.2.The results of mice acute toxicity by oral administration test,gentictoxicity(Ames test,mice bone marrow polychromatic erythrocyte micronucleus test,mice sperm abnormolity test in mice),teratogenicity test and ninety days feeding test indicated that under the condition of this experiment, Flos Puerariae groups were no significant different compared with the model group.Conclusion:Flos Puerariae had a protective effect on alcohol liver injury in rat. The results indicated that under the condition of this experiment, the Flos Puerariae was not any toxicity.