Clinical And Experimental Study On Treatment Of Colles’ Fracture Early Muscle Injury By Fresh Medicine Shuangbai Ointment

ObjectiveShuangbai Ointment scattered topical treatment of fractures of the early muscle injuries has exact curative effect, has been widely used in clinic. This topic on the basis of predecessors’ research experience is summarized, Change the main ingredients of Shuangbai Ointment like Chinese rhubarb、 Oriental arborvitae and mint to be used in the fresh, to made from fresh medicine Shuangbai Ointment, to observe the efficacy and safety of fresh medicine Shuangbai Ointment in the topical treatment of Colles’ fracture early muscle injuries. Through randomized controlled animal study to study the anti-inflammatory effect of Shuangbai Ointment and preliminary discuss its possible mechanism of the treatment of acute soft tissue injury. In order to provide effective method to treat the fractures of the early muscle injuries, provide scientific basis for the application and extension of Shuangbai Ointment.Methods(1) literature summary:Through consulting a large number of ancient and modern Chinese and foreign literature, Sorting and fracture early muscle injury, the paper summarized the research progress of Chinese and western medicine, the research work of Shuangbai Ointment and fresh medicine. (2) clinical research:a prospective randomized controlled clinical trials research method, will be included in the standard Colles’ fracture early muscle injury cases, After manual reduction small splint external fixation treatment were randomly divided into blank group, Shuangbai Ointment group, fresh medicine Shuangbai Ointment group,30 cases in each group. Blank group, chitin, Shuangbai Ointment and fresh medicine Shuangbai Ointment group respectively for the Shuangbai Ointment, fresh medicine Shuangbai Ointment topical treatment,1 times a day, each time the medication time not more than 9 hours, continuous treatment for 1 week. Observation record groups of patients before, chitin, chitin,1,4, and 7 days after the main clinical symptoms and signs and adverse reactions. (3) Experimentation:①Anti-inflammatory pain experiment:Take 50, kunming mice were randomly divided into blank group, helped him Lin, Shuangbai Ointment and fresh medicine Shuangbai Ointment parker paste, high dose group, each group of 10, only the type back shaving, blank group with vaseline, each corresponding outside coated dosage, dosing 7 days in a row. By xylene induced ear swelling test, carrageen glue to foot metatarsus swelling test, glacial acetic acid body torsion test, pain caused by hot plate test and tail pain of field instrument tail shrinkage test to study the anti-inflammatory analgesic action of fresh medicine Shuangbai Ointment. ②Acute soft tissue injury repair experiments:Establishing the model of acute soft tissue injury in rats were randomly divided into model group, voltaren group, Shuangbai Ointment and fresh medicine Shuangbai Ointment parker paste, high dose group, each group of 10, another 10 to build mould as blank control group. Model group with vaseline, more than the corresponding external treatment, treatment group for 7 days in a row. Respectively before the observation, chitin, chitin,1, 4, and 7 days after injury in rats and assess leg injury symptom index. For 7 days after anesthesia and the hemorrheologic changes of abdominal aorta in pursuance of observation, The determination of serum interleukin-1 beta, interleukin 6 and tumor necrosis factor alpha, prostaglandin E2 and inflammatory factors, such as c-reactive protein levels. Executed based in rats after injury, determination of tissue damage of nitric oxide and the content of superoxide dismutase (sod), muscle tissue pathological morphology observation damage.Results(1)literature summary:Summarizes the research progress of Chinese and western medicine of fracture early muscle injury and Shuangbai Ointment and fresh general situation of the research in Chinese medicine, in order to further research of early fracture muscle injury treatment provides a beneficial train of thought and research basis. (2) clinical research: Shuangbai Ointment and fresh medicine Shuangbai Ointment group of patients’ pain, tenderness, swelling and ecchymosis were improved significantly. Which in terms of the improvement of the pain, swelling, ecchymosis, fresh medicine Shuangbai Ointment group is better than the Shuangbai Ointment parker, parker was statistically significant difference (P<0.05), while in tenderness and overall efficacy were similar in both groups, there was no statistically significant difference (P>0.05). Fresh medicine Shuangbai Ointment parker found no obvious side effects. (3)Experimentation:①Anti-inflammatory pain experiment:Fresh medicine Shuangbai Ointment can decrease the xylene induced ear swelling in mice induced with carrageen glue mouse foot metatarsus swelling degree; Fresh medicine Shuangbai Ointment can increase the mice pain threshold, reduce the number of the body torsion induced by acetic acid, extend the effect the body torsion induced by hot plate and tail pain of field instrument to mice tail of the incubation period. ②Acute soft tissue injury repair experiments:Fresh medicine Shuangbai Ointment, high dose group of rats damage symptom index and voltaren quite Lin group, manifest is lower than the model group, the difference was statistically significant (P<0.05 or P<0.05). Fresh medicine Shuangbai Ointment, high dose group of rats serum IL-1 beta, IL6, TNF alpha, PGE2, CRP and NO content in serum was significantly lower than the model group and Shuangbai Ointment group, the difference was statistically significant (P<0.05). Fresh medicine Shuangbai Ointment can improve SOD content of tissue damage. Fresh medicine Shuangbai Ointment, high dose group of hemorheology index is close to blank group, significantly lower than the model group, the difference was statistically significant (P<0.05). Fresh medicine Shuangbai Ointment, high dose group of rats hit center muscle damage degree was significantly lower than model group, the difference was statistically significant (P<0.05).Conclusion1. Fresh medicine Shuangbai Ointment ointment topical treatment Colles’ fracture early reinforcement effect is good, and found no significant side effects.2. Fresh medicine Shuangbai Ointment can effectively improve the rat model with acute soft tissue injury injured limb muscle injury symptom index and hit the center of damage degree.3. Fresh medicine Shuangbai Ointment has good anti-inflammatory analgesic action, the action mechanism of the treatment of acute soft tissue injury is by regulating serum IL-1 beta, IL-6, TNF alpha, PGE2, CRP and loss of the expression of NO and SOD in tissue and improve hemorheology index, improve symptoms such as pain, swelling and ecchymosis.