Experimental Study Of Vascular Effects Of Moxibustion Mediated By TRPV1

Objective:This paper aims to study the vascular effects of moxibustion which mediated by TRPV1. We use moxibustion as the main intervention, to research normal rats’ local skin microcirculation blood flowmotion under different thermal stimulation, the correlation of moxibustion activate TRPV1 and local dermal tissue mast cells、mesenteric artery and vascular endothelial related factors like CGRP and eNOS, and further explore the vascular effects and mechanism of moxibustion.Methods:The study included theoretical and experimental research. The main contents of the theoretical study are to introduce the theory of vascular endothelium and vascular microcirculation system, and collecting and organizing literature of clinical and experimental study of modern domestic and foreign local thermal stimulus, and its vascular endothelial function and microcirculation. Experimental studies include:1, Using advanced topical skin microcirculation detection technology to compare influence local skin microcirculation blood flow under different temperature thermal moxibustion stimulation in normal SD rats moxibustion acupoints (Zusanli, ST-36); 2, compare different ways (mild moxibustion and scar moxibustion) of thermal stimulation on normal SD rats and its moxibustion affection on local dermal tissue morphology and function. Evaluate the effects of thermal stimulation on Guanyuan (BL26) acupoint skin to observe differences in mesentery artery TRPV1 protein and mRNA expression under different thermal stimulation, radioimmunoassay changes in eNOS and CGRP content in blood plasma, conclude intrinsic link between moxibustion thermal stimulation and moxibustion local skin and blood vessels in vivo correlation.Results:1, In the first experiment, compare lower limb microcirculation between two groups of rats can be concluded from the heating point of view, local skin blood flow volume in the moxibustion group has greater volatility, difference between two groups not statistically significant (P>0.05); from a temperature point of view,43℃ group has greater perfusion variation width, difference between two groups was not statistically significant (P>0.05); from the point of view in duration of effect, the temperature effect of the duration of the moxibustion group longer than the control group, the difference was statistically significant (P<0.05); 2, in the second experiment, compared with the control group, TRPV1 protein levels in mild moxibustion group was higher than the blank group, the difference was statistically significant (P<0.05), TRPV1 protein levels in scarring moxibustion group were lower than the control group, the difference was statistically significant (P<0.05); mRNA expression of mild moxibustion group and scarring moxibustion group both decreased, the difference was statistically significant (P<0.05); compared with the control group, mild and scarring moxibustion groups’ CGRP content in plasma of rats both decreased, the difference was statistically significant (P<0.05); mild moxibustion group’s eNOS content has declined compared to blank group, the difference was statistically significant (P<0.05); 4, mast cells of mild moxibustion group (10.24±0.44), mast cells of scarring moxibustion group (12.31±0.52), both increased significantly (P<0.05) compared with the normal group (6.50±0.76).5, correlation of TRPV1 protein、mRNA levels and the amount of moxibustion local skin mast cells changes with moderate positive, the difference was statistically significant (P<0.05);Conclusion:1, through different stimuli ways of moxibustion operation, activate TRPV1 channel can produce different vascular effects; 2, moxibustion activate TRPV1 channel by activating the skin mast cell, also can cause TRPV1 vascular effects.