The Study Of Lian Hua Qing Wen Capsule Components Against Influenza Virus On Pharmacological Characteristics

ObjectiveTo study Lian hua qing wen capsule components(Ma xing shi gan tang and Yin qiao san) against influenza virus on pharmacological characteristics.Methods1)The effect of Ma xing shi gan tang(MXSGT) and Yin qiao san(YQS) against influenza virus was tested using cytopathic effect(CPE) assay and MTT assay in Madin-Darby canine kidney;2)GAPDH as a reference gene,the expression of cytokines and chemokines which were induced by influenza virus was determined by real-time RT-PCR and ELISA; 3) The expression of NF-KB-p65 was tested by western blotting;4) Model mice by influenza virus A/PR/8/34(H1N1) Were randomly divided into seven groups:the nomal group,model group,oseltamivir group (60mg/kg/d),MXSGT high dose(1000mg/kg/ d),MXSGT medium dose(500mg/kg/d),YQS high dose(800mg/kg/d),YQS medium dose (400mg/kg/d).The momal condition and pulmonary index were observed.Results1)MXSGT is effective in inhibiting different subtypes of influenza virus strains(H1N1、H3N2、H6N2、H9N2、FluB) while YQS is ineffective in inhibiting cytopathic effect of influenza virus in vitro;2)The two prescriptions can regulate the expression of cytokines and chemokines that influenza virus induced, including IP-10、IL-6、IL-8、MIG、 CCL5、TNF-α,specially IP-10、IL-6(p<0.001);3)MXSGT(3～0.38mg/mL) can inhibit the phosphorylation of NF-KB-p65, YQS(2～0.25mg/mL) also can inhibit the phosphor ylation of NF-KB-p65;4) The high and medium dose of MXSGT had remarkable decreasing effect on the increase of pulmorary index of mices with viral infection by influenza virus A/PR/8/34(H1N1)(p<0.001);The medium dose of YQS had remarkable decreasing effect on the increase of pulmorary index of mices with viral infection by influenza virus A/PR/8/34(H1N1)(p<0.01),the high dose had no the same effect.ConclusionThis study shows MXSGT not only has an direct antiviral activity,but also play a role in anti-inflammatory effect in vitro.MXSGT has inhibitory effect on the pulmonary impair ment by influenza virus in vivo. Possible mechanisms are MXSGT inhibit the expression of NF-KB-p65 signaling pathway. YQS has not an direct antiviral activity,but play a role in an ti-inflammatory effect in vitro.YQS also inhibit the expression of NF-KB-p65 signaling pathway in vi tro.YQS has inhibitory effect on the pulmonary impairment by influenza virus in vivo.The mechanisms of YQS needs further study.