Investigating The Effects Of Total Flavonoids Of Litchi Chinensis Sonn On Cell Proliferation And The Expression Of Nf-кB And α-SMA In Activated Human Hepatic Stellate Cell

Objective: To study the effects of total flavone of semen litchi(TFL)on cell proliferation,apoptosis and the expressions of nuclear fact or-кB(NF-кB), α-smooth muscle actin(α-SMA)in transforming g rowth factor-β1(TGF-β1) induced human hepatic stellate cell. Methods: HSC-LX2 cells were cultured in vitro, using TGF-β1 stimulating 24 h, then gave different concentrations of TFL for different length of time. MTT method was used to detect the effect s of TFL on HSC-LX2 cell proliferation. Fluorescence staining was used for observing the morphological changes of apoptotic cells. R T-PCR was used to detect the expression levels of NF-кB and α-S MA mRNA. The levels of NF-кB and α-SMA proteins were detecte d by Western blot assay. Results: 1. MTT method showed that TF L could inhibit TGF-β1 induced HSC-LX2 cell proliferation in a timedependent manner. The effect of 48 h treatment was the most signifi cant and its IC50 was 302μg/ml. 2.We could observe the morpholo gical changes of apoptotic cells, such as nuclear fragmentation, kary opyknosis, chromosomal condensation, cell detachment and so on by fluorescence staining. 3.The expression levels of NF-кB,α-SMA m RNA were both decreased among the TFL treatment Groups. Comp ared with control group, the expression levels of NF-кB,α-SMA we re significantly lower in 300μg/ml and 600μg/ml drug concentration group(P<0.05). 4.The expression levels of NF-кB,α-SMA protei ns were both decreased in a concentration-dependent manner among the TFL Groups. Compared with control group, the expression lev els of NF-кB,α-SMA were significantly lower in 300μg/ml and 600μg/ml drug concentration group(P<0.05). Conclusion:1.In vitro,TFL may inhibit the proliferation of TGF-β1-induced HSC-LX2,so as to prevent the formation of hepatic fibrosis, which may be asso ciated with reducing the expression of NF-кB and promoting cell apoptosis. 2.With the increase of TFL concentration and action time,the inhibitory effect of TFL on the proliferation of HSC-LX2 was more notable, and the effect of anti hepatic fibrosis was more significant.