| Objective: Observe the influence of the vitamin D and vitamin AD on the concentration of serum IL-33, IgE and bronchoalveolar lavage fluid cell counts, concentration of IL-33 and changes in lung tissue in a murine model with asthma. Explore the role and mechanisms of vitamin D and vitamin AD supplementation in a mouse model with asthma, and understand the role of IL-33 in asthma.Methods: 48 BALB / c mice which were 4 to 5 weeks old, female and clean were randomly divided into normal control group A, asthma control group B, asthma and vitamin D group C(two week before sensitization), asthma and vitamin D group D(sensitization), asthma and vitamin D group E(after 7 days of challenged), asthma and vitamin AD group F(two week before sensitization), asthma and vitamin AD group G(sensitization), asthma and vitamin AD group H(after 7 days of challenged). BALB / c mice were sensitized and challenged with ovalbumin to establish the asthmatic model. Group C, group D and group E were respectively fed vitamin D through gavage two week before sensitization, sensitization and after 7 days of challenged. Group F, group G and group H were respectively fed vitamin AD through gavage two week before sensitization, sensitization and after 7 days of challenged. Group A and group B was controlled with NS. ELISA was used to detect serum IL-33, IgE and bronchoalveolar lavage fluid IL-33 concentrations, BALF eosinophil count proportion by Wright-GMS, HE staining of lung tissue.Results: 1) BALF and serum concentrations of IL-33: the serum concentrations of IL-33 in group C are the lowest except group A(P<0.05). The BALF concentrations of IL-33 in group B was significantly higher than in group A(P <0.05), but there were no differences compared with other groups. 2) IgE concentration: in asthma groups, group C [(12.25 ± 1.19) ng / mL] was the lowest compared with other groups(P <0.05). Group D [(14.17 ± 1.11) ng / mL] was lower compared with group G [(16.86 ± 2.03) ng / mL], comparison between the groups was different(P <0.05). 3) cell counts and EOS(%) in BALF: The cell counts and EOS% in group C were significantly lower than other asthma group(P <0.05). 4) lung tissue: There was no obvious inflammatory cells in group A.Group B showed increased bronchial mucosal folds, lumen stenosis, a large amount of inflammatory cells infiltration around the lumen, the smooth muscle and basement membrane thickening. In group C, no obvious epithelial cells and smooth muscle proliferation, no obvious wall thickening, less inflammatory cell infiltration, but if compared with group A, mentioned above were slightly increased. In group D, E, F, G and group H, bronchus spasm mild, epithelial cells and smooth muscle hyperplasia mild, slightly thickened basement membrane, less peribronchial inflammatory cell infiltration.5) serum IL-33 level and EOS% of BALF were positively correlated(r=0.878,P<0.01), serum IL-33 level and serum IgE level were positively correlated(r=0.549,P<0.01), BALF IL-33 level and EOS% of BALF were positively correlated(r=0.627,P<0.01), BALF IL-33 and serum IgE level were positively correlated(r=0.555, P<0.01), the difference was statistically significant.Conclusions: 1) Adequate intervention with vitamin D in the early life could alleviate the inflammation in the lung tissues, reduce the secretion of IL-33, IgE and eosinophil infiltration. 2) IL-33 may play an important role in the pathogenesis of asthma, the concentration of serumIL-33 may be a mark of the severity of asthma, and had a positive correlation with IgE and EOS% level.3) Adequate intervention with vitamin D is more effective for improve lung inflammation in mice with asthma than vitamin AD. |
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