The Experimental Study Of Anagenetic Fibula After Removing Young Rabbits

Objective:To investigate the difference between anagenetic fibula with normal fibula in mechanical parameters, osteogenesis and tissue morphology,to proive a evidence to autologous fibular graft in clinic.Methods:A total of 20 3-month New Zealand white rabbits, divide two groups (retaining the periosteum and not retaining the periosteum), made the fibula bone defects with 1.5 cm length, X-ray was used to observe the healing of the fibula (half month, one month, two months). All the rabbits were killed after two months (the group of retaining the periosteum), tooked the anagenetic fibula and normal fibula on the side at the same location (1.5cm).The three-point bending test of the diaphysial fibula was used to test the mechanical properties, the alkaline phosphatase staining to gauging the osteogenesis and the HE staining to check the tissue morphology.Results:Observation of radiographs of all animals:At half month after surgery a few new bone were forming at two ends of resection area. At one month after operation new bone growing in donor site which almost connecting the cortex.At two month postoperative new fibula were completely formed with the cortex bone were intergrated. The three point bending test of fibula showed: there was no statistically significant difference in the elasticity flexibility, the failure Load, the maximum Load, the maximum flexibility and the Youning’modulus between anagenetic fibula with normal fibula (P> 0.05); alkaline phosphatase staining:both two group showed the APL are positive along the side of the interior of the cortex bone.HE staining:the structure of cortex bone are intergrate and the haversian canal are normal comparing with the matched group.Conclusion:Fibula were regenerated successfully after removing which retaining the periosteum. Comparing with the contralateral fibula, there were no remarkable differences in mechanical parameters, osteogenesis and tissue morphology. In our clinical work, persisting the periosteum can be regenerated fibula in situ when surgerying, which would has little effect on the donor site.