Study On The Relationship Between Human Cytomegalovirus Infection And Essential Hypertension

Background and ObjectiveAs most common chronic disease, Hypertension is an independent risk factor for atherosclerosis. The damage of heart, brain, kidney and other target organ caused by hypertension has become the main factors which not only influence the quality of patients’ life, but also brings heavy economic burden to society and family. With the development of the cardiovascular epidemiology and evidence-based medicine, the prevention and control of hypertension has made certain progress in China in recent years. However, the task to prevent and control hypertension is still arduous. The pathogenesis of hypertension is not clear at present. With the deepening study of pathology and path physiology, people find that inflammation plays a very important role in the development of hypertension. Many studies have found that HCMV can induce apoptosis of vascular endothelial cells, proliferation of vascular smooth muscle cell, which cause the intimal thickening, vascular lumen narrowing, and the final result is lead to atherosclerosis and other vascular lesions. Furthermore, the correlation research between HCMV infection and the incidence of hypertension is not many. As the closely linked between the arteriosclerosis and the development of hypertension, the purpose of this study is comparing the HCMV infection rate and plasma neutralizing antibodies levels between the hypertensive patients and healthy people, to discuss the relationship between HCMV infection rate and high blood pressure preliminarily.MethodsThe diagnostic criteria of hypertension is according to "guidelines for prevention and treatment of hypertension China (2013 Edition)". Select the essential hypertension (grade 1,2) patients in total of 60 cases who came to our hospital from January 2014 to December2014 as experimental group. Select the healthy persons in total of 60 cases who came to our hospital for medical examination at the same time as control group. Blood pressure was measured in two groups of subjects. Fasting blood samples were collected from the subjects of the two groups and the following tests were conducted.1. Fasting blood glucose, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol, aspartic acid amino transferase, alanine aminotransferase, urea nitrogen, creatinine in blood samples of patients were detected by automatic biochemical analyzer, and the results were recorded in detail.2. Determine the HCMV DNA in blood samples by the human cytomegalovirus nucleic acid fluorescent quantitative detection kit.3. The HCMV IgG and IgM in blood samples were determined by human cytomegalovirus IgG or IgM ELISA assay kit and the test results were record detailed.4. The HCMV specific neutralizing antibody of the HCMV IgG positive blood samples were detected at the same time and the test results were record detailed.5. Use SPSS 19.0 statistical software package for data analysis. Using the mean ± standard deviation (X ±S) expressed the data if measurement data obeyed the normal distribution. Using the median ± four percentile interval (M ± Q) expressed the data, if measurement data did not obey the normal distribution. The t-test was used for the measurement data. The chi square test was used for the statistical comparison of count data. All of the test hold a=0.05 as the standard test.p<0.05 that the difference was statistically significant.Results1. Comparison of general information in the two groups:Age, gender, blood pressure, The fasting blood glucose, total cholesterol, triglyceride, low density lipoprotein cholesterol, high density lipoprotein cholesterol, aspartic acid amino transferase, alanine aminotransferase, urea nitrogen and creatininein were compared respectively to hypertension group and control group. In result, In hypertension group and control group study, the differences in age, gender, high density lipoprotein cholesterol, aspartic acid amino transferase, alanine aminotransferase, urea nitrogen and creatininein had no statistical significance (P> 0.05).However, Systolic blood pressure, diastolic blood pressure, The fasting blood glucose, total cholesterol, triglyceride and low density lipoprotein cholesterol of the hypertensive patients was higher than that of the control group patients. This indicates that there are significant differences between the two groups(P<0.05).2. Comparison of blood DNA HCMV in the two groups:The HCMV DNA detection rate in hypertension group was 71.67%. The HCMV DNA detection rate in control group was 55.00%. The detection rate of the hypertension group was higher than that of the control group (P<0.05). There is significant difference between the two groups3. Comparison of plasma IgG HCMV and IgM in the two groups:The positive rate of HCMV IgG and IgM in the hypertension group was 70.00% and 41.67% respectively. The positive rate of HCMV IgG and IgM in the control group was 56.67% and 33.33% respectively. The positive rate of HCMV IgG in the hypertension group was higher than that of control group, the two groups had significantly statistical difference (P<0.05). However, the positive rate of HCMV IgM in the two groups had no statistical difference (P>0.05).4. Comparison of HCMV specific neutralizing antibodies in plasma of the two groups:In the HCMV IgG positive blood samples, the 50% HCMV specific neutralizing antibody titer of the hypertension group was (37.55±20.06), and the HCMV specific neutralizing antibody titer of the control group was (59.69±24.32). The 50% HCMV specific neutralizing antibody titer of the hypertension group was significantly lower than that of control group, the two groups had significantly statistical difference (P< 0.05).Conclusion1. The abnormal rate of blood glucose levels, cholesterol levels, low density lipoprotein cholesterol and triglyceride levels and the HCMV infection rate of hypertensive patients was higher than that of the healthy people.2. The positive rate of HCMV IgG in the hypertensive patients was significantly higher than that of healthy people. However, the positive rate of HCMV IgM between the hypertension patients and the healthy population had no difference.3. The HCMV specific neutralizing antibody titers of the hypertension patients was significantly lower than that of healthy people.4. Infection of HCMV is a risk factor for hypertension. HCMV can be detected in the early prevention and early diagnosis of high blood pressure events.