Research On Interaction Between Human Cytomegalovirus Helicase And Cellular Factor Snapin&the Epidemiological Investigation Of Hand,Foot, And Mouth Disease In Nanchang,2010

Controlled regulation of genomic DNA synthesis is a universally conserved process for all herpesviruses, including human cytomegalovirus(HCMV), and plays a key role in viral pathogenesis, such as persistent infections. HCMV UL105is believed to encode the helicase of the DNA replication machinery that needs to localize in the nuclei, the site of viral DNA synthesis. No host factors that interact with UL105have been identified, In this study, we show that UL105specifically interacts with Snapin, a human protein that is predominantly localized in the cytoplasm and associated with cellular vesicles. UL105was found to interact with Snapin in both the yeast two-hybrid screen and coimmunoprecipitation experiments in HCMV-infected cells. Our results provide the first direct evidence to suggest that Snapin interacts with UL105and alters its cellular distribution, leading to modulation of viral DNA synthesis and progeny production. Our study further suggests that modulation of the cellular distribution of viral helicase by Snapin may represent a possible mechanism for regulating HCMV genomic DNA synthesis, a key step during herpesvirus lytic and persistent infections.Furthermore, the outbreaks of hand, foot and mouth disease (HFMD) in China have affected millions of people recently. HFMD potentially lead to a variety of neurogenic complications and become a threat to the newborns health. Understanding the epidemiology of HFMD and EV71infection is essential for controlling outbreaks, In this report, we studied an outbreak of HFMD in Nanchang by both epidemiological and laboratory analyses. The epidemiological study shows that the age group of1-3years old children containing the highest number of cases. Laboratory studies suggest EV71was a common enterovirus found in Nanchang. Phylogenetic analysis of the EV71strains isolated from Nanchang indicated that these strains belong to the C4subgenotype commonly found in Fuyang HFMD outbreaks in2008, but contain several variations. For the first time, Our study described the characteristics of the epidemiology of HFMD in Nanchang in2010and proved that the genotype of EV71strains circulating in Nanchang were C4. Our study must have guiding significance in the control and prevention of HFMD and EV71infection in high-risk people.