Study On Chemical Constituents Of Aconitum Leucostomum Worosch

Aconitum leucostomum Worosch is Ranunculaceae Aconitum perennial herb, which mainly produced in Xinjiang province. Aconitum leucostomum Worosch is used as a substitute for Aconiti kusnezoffii Radix in the Kazakh nationality,which is spicy, hot, bitter, toxic, commonly used to arthralgia due to wind cold dampness and other diseases, is one of the northwest minority commonly used as folk medicine. It contains ditepenoid alkaloids, fatty acids, amino acids and so on. Some of these compounds showed analgesic, anti-inflammatory, anti-tumor and other aspects. For the the further study of chemical constituents and biological activity in Aconitum leucostomum Worosch, we isolatied and identificated the Aconitum leucostomum Worosch, and did some study on the pharmacological activity of isolated compounds.In this paper, twelve coumpounds were isolated from Aconitum leucostomum Worosch by a variety of means of separation, including silica gel column chromato-graphy, recrystallization and so on. Their structures were identified as lappaconitine (1), dehydroacosanine (2), ranaconitine (3), N-methylphthalimide (4), songorine (5), aconitine (6), mesaconitine (7), deoxylappaconitine (8), spicatine A (9), indaconitine (10), puberuline C (11) and chasmaconitine (12). Compounds 6,7,8,9,10,11 and 12 were isolated from this plant for the first time, compound 4 was isolated from plant for the first time.In this paper, the anti-inflammatory activity of some compounds were determined, and the organ coefficient of the mices which took the compounds were determined. Studies have shown that of the compounds have anti-inflammatory effect; mice taking the compounds caused organ coefficient of variation were not obvious.