Study Of Experimental Diabetic Periodontitis-associated Bone Loss In Rats

Objective: To investigate the mechanism of periodontitis—associated bone lossin diabetic rats．Methods：Fifty-two eight—week—old male SD rats were randomly divided intothree groups：diabetic periodontitis group (DP)，periodontitis group(P)and normalcontrol group(N)． Diabetic rats were induced by intraperitoneal injection ofstreptozotocin(STZ)．Periodontitis was initiated by ligating floss around maxillarysecond molars and oral inoculation with pefiodontM pathogen Porphyromonasgingivalis． Two or four or six weeks later， animals were sacrificed and thespecimens were prepared for histological analysis．Assessment w∞carried out byusing different visual and histological approaches． Visual assessment includedalveolar bone loss(ABL)．Histological examination included osteoclast numbers inthe inflammatory area． Statistical significance Was determined by one—wayANOVA with significance set at P<0.05．Results：The sequence of mean alveolar bone loss among each group Was groupDP>group P>groupN．Inter—group analysis revealed higher osteoclast numbers inthe inflammatory area of group DP and group P when compared with groupN(P<0.05)． Compared with group P， group DP had more higher osteoclastnumbers(P<0.05)．Conclusion： Diabetes Can increase the severity of periodontitis． Acceleratedalveolar bone loss Was observed in diabetic rats．Increased osteoclast numbers in theinflammatory area in diabetes may contribute to the exaggerated destruction ofalveolar bone． Diabetes may enhance periodontitis·associated bone loss byincreasing the formation of osteoclasts in the periodontal tissue．...