Effect Of Resistin On The Permeability Of Endothelial Cells

Resistin, also known as the FIZZ3, is an adipocyte-specific secretory factor. According to a large amount of preview studies, resistin has a significant association with insulin resistance as well as high blood glucose.Small molecular substance can pass through vessels into tissues by transportation or paracellular flux which is controlled by tight junctions among vascular endothelial cells. Tight junctions have two important functions:First, they can prevent large molecular substance and irons getting through them, leading the vascular endothelial cells to transport materials in a selective and more accurate way. Second, they inhibit the movement of proteins that locate on the apical and basolateral surface, thus the receptor-mediated endocytosis and exocytosis will be preserved.Here, we exploit EA.hy 926 cells that act as the model of vascular endothelial in vivo to study the relationship among resistin, insulin resistance and high blood glucose. Firstly, we apply transwell cell culture system, blood glucose monitor and ELISA to measure the effect of resistin on the glucose and insulin’s transportation in vascular endothelial monolayer. Also, with the help of RT-PCR, western blot and wound assay, we give a deep sight on the potential pass way how resistin regulates the process of glucose and insulin’s transportation. The following statements are the results we got in the experiments.1.After treating with resistin, we measure the concentration of glucose and insulin in the lower compartment and intracellular, then we find that resistin can restrain EA.hy 926 cells absorbing and transporting glucose as well as insulin.2.EA.hy 926 cells treated with resistin facilitate cell proliferation but cannot affect tight junctions and this proves that there is no relationship between cell proliferation and tight junctions.3.Resistin can down regulate the expression of INSR and GLUT1 mRNA which result the dysfunction of vascular endothelial. Also LPS, an inflammation factor, can incite the similar phenomenon as resistin does, meaning that inflammation also regulates the permeability of EA.hy 926.