| Obeject:1. Clinical Study:To investigate the relationship between testosterone and lipids and other risk factors for coronary heart disease in middle-aged and elderly men.2. Animal Study:Middle-aged male rat model were established by fat diet, and then did castration surgery and complemented testosterone. So middle-aged male rats of different degree of partial deficiency of testosterone were established, and then observe the effect of testosterone concentration changes of lipid.Methods:1. We selected 242 middle-aged and elderly men (40-79 years, mean age 58.23±13.49 years) from inpatient and outpatient medical examination of our hospital. The following information were collected:height, weight, blood pressure, body mass index (BMI), total testosterone, total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL -C), low-density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG) and fasting insulin (Fins) levels and insulin resistance index (HOMA-IR).2.①High-fat diet was reared for 50 SD male rats aged 10 months (equivalent to middle age men) for 6 weeks, and of which 47 survived, successfully established animal model of hyperlipidemia.② In which 41 rats underwent castration randomly,6 sham castration as control group. After 12 days adaptive feeding, ovariectomized rats survived 24, and then randomly divided into four groups:complete lack of testosterone group(n= 6, accepted the corresponding amount of medical corn oil hypodermic injection), low dose testosterone group (n=5, accepted 1mg/kg of testosterone propionate hypodermic injection), middle dose testosterone group(n=6, accepted 5mg/kg of testosterone propionate hypodermic injection) and high dose testosterone group(n=7, accepted 25mg/kg of testosterone propionate hypodermic injection). Control group was accepted the corresponding amount of medical corn oil hypodermic injection. Testosterone supplemented for 8 weeks. Detect testosterone and blood lipid levels in peripheral blood to observe the effect of testosterone on the changes in the concentration of lipid metabolism.Results:1. The relationship between testosterone and lipids in middle-aged and elderly men: testosterone were negatively correlated with TG and TC (all P<0.05), and testosterone were not associated with HDL-C and LDL-C (all P>0.05).2. The relationship between testosterone and other risk factors for coronary heart disease in middle-aged and elderly men:testosterone were negatively correlated with with age and BMI (all P<0.05), and testosterone were not associated with SBP, DBP, FPG, Fins and HOMA-IR (all P> 0.05).3. Effect of high fat diet on lipid metabolism in middle-aged male rats:after high-fat diet for 6 weeks, serum TC, TG and LDL-C levels were higher than before high-fat diet, HDL-C levels lower (all P<0.01). Fat model successfully established.4. Different concentrations of testosterone on testosterone levels in castrated male aged rats:testosterone levels of testosterone middle dose group and control group were similar (P> 0.05). Complete lack of group and low dose group testosterone levels were lower than the control group (P<0.05). High dose group testosterone levels were significantly higher than the control group (P<0.05).5. Effect of different concentrations of testosterone in aged male castrated rats blood lipid levels:complete lack of testosterone group and low dose group TC and LDL-C levels were higher than the control group (P<0.05); high dose group and middle dose group TC and LDL-C levels were lower than complete lack of group. Each group TG and HDL-C levels were not statistically significant (all P> 0.05).Conclusion:1. With increasing age, testosterone levels gradually decrease. With the decrease of testosterone levels, TG, TC and BMI increase in middle-aged and elderly men.2. Middle-aged and elderly male rats’ serum testosterone levels could adjust serum cholesterol levels:TC and LDL-C levels could increase with testosterone lack of severity, at the same time high levels of testosterone could decrease TC and LDL-C levels. |
