Study On The Biological Function Of Leptin Receptor In Hela Cells

Cervical cancer is one of the most common malignant tumors in the female reproductive system. It has been found that leptin receptor (CD295) in hepatocellular carcinoma, breast cancer and astrocytoma had an effect on biological characteristics of cancer cells such as migration, colony-forming ability, tolerance to cancer drugs [1-4].This study mainly investigated the effect of CD295 protein on the biological function of HeLa cells. The results showed that CD295 was a surface protein of HeLa cells by flow cytometry and immunofluorescence detection; therefore, we obtained CD295+HeLa cells by flow cytometer using CD295 marker. HeLa cells were transfected with CD295 interference plasmid vector or empty vector SD1211. Then, CD295 interference plasmid vector (pGCsi-CD295) and empty vector stably transfected cell line (control) were selected by G418.Finally, we obtained four cell lines:CD295+HeLa cells, HeLa pGCsi-CD295 cells, untreated HeLa cells and empty vector stably transfected cells (control), and explored the difference of biological function between them, including the clone forming ability, migration ability, cell invasion, sensitivity to paclitaxel (PTX) and cell cycle distribution. The results showed that CD295 in HeLa had significant effects on the ability of cloning, the ability of migration and invasion, the ability to tolerate tumor drug PTX and cell cycle distribution. So we suggested that the high expression of CD295 in HeLa is related to cancer sternness.