The Effects Of General Flavonoids From Alpinia Officinarum Hance On Cell Proliferation, Apoptosis And Expression Of Human Cervical Cancer Cell Lines

Objective: To investigate the effect of general flavonoids(GFs) isolated from Galangal root on the morphology, proliferation and apoptosis of SiHa cervical cancer cells, and its association with the expression level of cancer stem cell marker expression, to reveal anti-cancer mechanisms of galangal GFs, and provide evidence for developing novel anti-cancer drugs based on glangal flavonoids. Methods: 1) The total flavonoids were isolated from Alpinia officinarum hance by gradual chemical extraction using ethanol, petroleum ether and chloroform; 2) The change in cell morphology in response to treatment of SiHa cervical cancer cells with different doses of galangal GFs were documented by inverted optic microscopy; 3) The change in cell viability after treatment of SiHa cell with galangal Gfs was measured by methyl thiazolyl tetrazolium(MTT). 4) Cellular apoptosis of SiHa cells in response to treatment with two different dosed of galangin GFs was detected by flow cytometry, setting DDP as a control; 5) The effect of galangin GFs on transcription level of cancer stem cell markers was determined in SiHa cells in response to treatment by fluorescent quantitative RT-PCR; 6) Experimental data is represented in mean + SD, and statistical analysis was performed by using SPSS software version 17.0, with statistical significance at P<0.05. Results: 1) With the treatment of SiHa cells with galangal GFs in the dose range of 0 ~ 400 g/ml, cell viability was decreased significantly(12%),(the IC50 values: 24 h, 127.393 g/ml; 48 h, 84.584g/ml; 72 h, 58.054 g/ml), in a dose and time dependent manner, and with a tendency in the inhibition of cell viability similar to positive control DDP; 2) According to the data of flow cytometry, the treatment of SiHa cells with galagal GFs could induce cellular apoptosis, among which the rate of early stage apoptosis was increased at doses from 50 and 100μg/ml(17.35% v.s. 22.4%), but this is much lower than that of positive control(60.4%); 3) The transcription of OCT and ALDH1A1 was significantly decreased in response to treatment of SiHa cells with galangal GFs(P<0.05), but no difference was found for TWIST1(P>0.05). Conclusion: 1) Galangal GFs can inhibit cell proliferation and induce cellular apoptosis in a dose and time dependent manner; 2) The treatment of SiHa cell with Galangal GFs may cause the downregulation of cancer stem cell markers, as a partial mechanism of the anti-cancer effects of the drug.