Screening And Identification Of Human Prostate Tissue Immunodominant Antigens

Prostatitis is a common disease of urology, and its pathogenesis is uncertain. In the recent years, the research results of different experimental autoimmune prostatitis (EAP) rodent models proved that autoimmune factor is one of important factors in the occurrence of prostatitis. A variety of autoimmune prostatitis associated antigens were identified from different rodent models of prostatitis, like prostate steroid binding protein (PSBP), prostatic acid phosphatase (PAP) and seminal vesicle secretory protein 2 (SVS2) and so on. However, little similar antigens were reported in human body. Therefore, screening and identification of human prostate tissue immunodominant antigens will play a significant role in the research of discovery of the autoimmune prostatitis associated autoimmune antigens. In this study, to obtain anti-human prostate tissue antigen-specific antibody, we immunized rabbits with human benign prostatic hyperplasia tissue homogenates proteins, and used the obtained antibodies to screening and identify immunodominant antigens in human prostate tissue by specific binding between antigen and antibody. We analyze the relevant information on immunodominant antigens in human prostate tissue through two-dimensional electrophoresis, mass spectrometry, and immunohistochemistry. After screening and identification,16 kinds of immunodominant antigens derived from human prostate tissue were found, and as far as we known which of the three proteins have been reported associated with prostate disease. Moreover, the purified immunodominant antigens can induce prostatitis in mice. The information about these immunodominant antigens provide a reference for further study of human autoimmune prostatitis associated antigens.