Study On Acute Toxicity And Reducing Blood Lipids Of Piperic Acid Hexamethylene Diamine

Objective: Through the acute toxicity test of mice, evaluation the safety of piperic acid hexamethylene diamine; use the rat model of hyperlipidemia, research effect of piperic acid hexamethylene diamine on glucose and lipid metabolism in rats and its mechanism, provide scientific basis for its medicinal value. Methods: 1.Pepper acid hexamethylene diamine 5g/kg dose, 0.6ml/20 g gavage volume, gavage and continuous observation 14 days, recorded adverse reactions in mice, anatomical observation of major organs organization changes in mice.2.78 male SD rats, after one week period of adaptation, determination of basal serum cholesterol(TC) and triglyceride(TG) level, then randomly selected 12 as the normal control group received regular diet, others as a model group fed high- fat diet. After 4 weeks of high- fat diet, the serum levels of TC and TG were detected again, the blood lipid levels were compared before and after the model was made, and the TC and TG were significantly increased to build the model successfully. The successful model of the rats according to the serum TC and TG levels stratified and randomized group high fat model group, simvastatin positive drug group(10mg/kg body weight) and PAH high dose(10mg/kg body weight), middle dose(5mg/kg body weight), low dose(2.5mg/kg body weight). Oral drug delivery for 4 weeks then the rats were deeply anesthetized to collect blood samples and test total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL-C), low density lipoprotein cholesterol(LDL-C), superoxide dismutase(SOD), the activities of glutathione peroxidase(GSH-PX)and the amount of malonaldehyde(MDA), fasting blood glucose(FPG) and insulin(FINS)were determined. At the same time, paraffin sections were made to observe the morphological changes of the liver tissues of rats in each group by light microscope.Results:1.Observed after 14 days, all the mice survived, and did not see any toxic effects, The main organs have no abnormal change.2.PAH middle dose and high dose group serum TC and LDL-C levels decreased significantly than the high fat model group(P<0.01), high dose group LDL-C level was more significant difference in positive drug group(P<0.01), PAH each dose group TG levels were significantly lower than the high fat model group(P<0.01), PAH low dose group HDL-C level were significantly higher than the high fat model group(P<0.01) and positive control group(P<0.05). 3.PAH each dose group MDA level were significantly lower than the high fat model group(P<0.01) and the high dose group was lower than positive drug group(P<0.05), PAH middle dose and high dose group the activity of SOD were significantly higher than the high fat model group(P<0.01), the high dose group were higher than the positive drug group(P<0.05), PAH middle dose and high dose group the activity of GSH-PX were higher than the high fat model group, the high dose group was more significant(P<0.01). 4. PAH each dose group FPG levels were significantly lower than the high fat model group(P<0.01), the middle dose group HOMA-IR level was lower than the high fat model group(P<0.05). 5. The middle dose group significantly improved liver cell edema, has certain protective effect on liver cells. Conclusion: Piperic acid hexamethylene diamine is low toxicity, can reduce blood lipids and blood sugar, improve insulin resistance, the mechanism may be related to antioxidant activity.