The Effect And Mechanism Of Misgurnus Anguillicadatus Lyophilized Powder In Weight Training And Exhaustive Exercise Induce Mice Liver Apoptosis

Objective: Applied biochemistry, immunohistochemical and cell morphology test the structure, function of liver tissue and liver cell apoptosis and expression of apoptosis factor, for freeze-dried loach powder drink improve exercise capacity and protect rat liver were discussed, the relationship between reveals its anti-fatigue effect and mechanism, provide experimental basis for the application of sports practice and establish the scientific theory.Methods: Screening of 40 male ICR mice, according to the weight stratified randomly divided into control group(C) quiet, pure training group(T), freeze-dried loach powder intervention group(N), freeze-dried loach powder intervention to swim training group(N), T and N group mice into the water depth is about 80 cm inside the swimming, the water temperature control in 29 ±3 ℃.Five days a week, a total of three weeks training.The first week is not weight, swimming for 30 minutes, in the second week of the tail root weight 2%, sport 40 minutes, the third week weight 3%, 50 minutes to sport.Starting from the second week of group C and group T filling and physiological saline, filling and homemade freeze-dried loach powder drink N group.2 days off after the training, T and N group the tail root weight 5% exhaustion exercise.24 h after exhaustion exercise group blood and liver tissue based.(1) the activity of serum ALT and AST.(2) the detection of liver SOD activity and MDA content.(3) HE staining to observe the liver tissue cell structure and the change of the form.(4) TUNEL method is used to observe the liver cell apoptosis.(5) immunohistochemical method to observe the antiapoptotic factor the BCL- 2 and apoptosis factors bax protein distribution.(6) Western Blot detection the BCL- 2 and bax protein content in liver tissue.Results:(1) N mice modeled after a length was 123.52 ± 51.65 min, group T the exhaustion time in mice was 55.86 ± 20.23 min, N groups of exhaustion exercise time is significantly longer than T group exhaustion exercise time(P<0.01).(2) The activity of serum AST and ALT activity compared with group C, group T are on the rise(P > 0.05), and N are close to C group;Compared with group T and group N ALT activity is on the decline, the AST activity decreased significantly(P < 0.05), and AST/ALT were significantly lower(P < 0.05).(3) The SOD activity of three groups were not significant differences(P > 0.05).MDA content in T group is significantly higher in the group C and N group(P < 0.01), SOD/MDA declined significantly in the C group(P < 0.05), a significant decline in the N group(P < 0.01);And the MDA content decreased significantly compared with group C N group(P < 0.05), SOD/MDA increased significantly in the C group(P < 0.05).(4) The liver tissue cells HE staining showed that group C liver cells structural integrity, no degeneration and necrosis;T group of liver cell swelling, clearance increases, the nucleus smaller, collect abbacy cell infiltration phenomenon.N group of liver cells structural integrity, clear outline clear, no degeneration and necrosis;Hepatic sinus congestion phenomenon, of the no law of the nuclei are round and neatly.(5) TUNEL test showed that the liver tissue of group C with a few nuclei were dyed deep brown, apoptosis index was 23.50± 7.50%.N and C groups were slightly more nuclei were dyed deep tan, apoptosis index was 28.33±9.48%.T group has a lot of hepatic cells have been dyed dark brown, apoptosis index was 31.5±6.38%. T group and N group has increased apoptosis, group T trend is bigger, weak N group.(6) Western blot test showed that compared with group C, N and T group the Bcl- 2 are on the rise and Bax protein expression quantity(P > 0.05), among which the Bcl- 2 N expression amount and the Bcl- 2 / Bax C and T group significantly increased(P < 0.05) and Bax protein expression quantity is has no significant difference in group C and group T(P > 0.05).Conclusion: Freeze-dried loach powder can very significantly resist sports fatigue, prolong mouse swimming exhaustion exercise duration.With the enhancement of liver tissue factor expression ability to resist free radical, stable apoptosis ratio, reduce rat liver tissue damage, make liver cell apoptosis trend abate, optimization of liver tissue structure and function.