| Objective: Chronic fatigue syndrome (CFS), is a debilitating and complex disorder characterized by profound fatigue that is not improved by bed rest and that may be worsened by physical or mental activity. Persons with CFS most often function at a substantially lower level of activity than they were capable of before the onset of illness. In addition to these key defining characteristics, patients report various nonspecific symptoms, including weakness, muscle pain, impaired memory and/or mental concentration, insomnia, and post-exertional fatigue lasting more than 24 hours. In some cases, CFS can persist for years. The cause or causes of CFS have not been identified and no specific diagnostic tests are available. Moreover, since many illnesses have incapacitating fatigue as a symptom, care must be taken to exclude other known and often treatable conditions before a diagnosis of CFS is made.Traditional Chinese Medicine think that liver-stagnation and spleen-dificiency is the main pathogenesis of CFS, but the roles of its molecular mechanism need further research. Replicated the model of CFS mice by binding and swimming in chilled water, the paper studied the injury by oxidative stress and the changes of surviving condition of liver cell and reseached its molecular mechanism; and studied the effect of Xiaoyaosan on liver injury reseached its molecular mechanism and provided the experimental evidence for clinical prevention and treatment of CFS.Methods: 1. Replicating and evaluating the mice model: Replicated the model of CFS mice was replicated by binding and swimming in chilled water. After 3 days' adaptively swimming(once one day, 30min one time), the model was been replicated in the late 12 days (binding on the morning and swimming on the afternoon). Losding amount of binding was added every 3 days, time is 1h, 1.5h, 2h, 2.5h respectively. Swimming was exhaustive training which was taken in water, 60cm depth, in a glass vessel (120cm×120cm×80cm). The model was replicated seccessful by evaluating the model mice' s states and the grades in step-down test and mice-tail-suspended test. 2. packet and treatmeng: 120 CFS model mice were divided into 4 groups. The mice in model control group were recovered naturally; in low-dose Xiaoyaosan group were taken Xiaoyaosan (0.2ml/10g) by gavage; in hige-dose Xiaoyaosan group were taken Xiaoyaosan (0.4ml/10g) by gavage; and in vitamine C group were take vitamine C (0.2ml/10g) by gavage. The blank control group contained 30 mice that matched to the mice in other groups with age and sex. All mice were take by gavage for 7 days (once every day). 3. target examintion: liver of every mice was taken into paraffin sections which were observed by microcope after HE; the content of malondialdehyde (MDA) was measured with thiobarbituric acid method and the activity of superoxide dismutase (SOD) with xanthine oxidase method; TdT-mediated d-UTP nick end labeling (TUNEL) was used to detect the apoptosis of liver cells.Results: The model of CFS mice was replicated successfully by binding and swimming in chilled water. Compared with blank controll group, there was not significant pathomorphologic observation in liver of the model mice, but the activity of SOD decreased obviously (p<0.01), the content of MDA increased significantly (p<0.01) and the apoptosis of liver cells was higher obviously (p<0.01). Compared with model controll group, the activity of SOD in liver of the mice of low-dose Xiaoyaosan group, hige-dose Xiaoyaosan group and vitamine C group increased obviously (p<0.01), the content of MDA decreased significantly (p<0.01) and the apoptosis of liver cells was lower obviously (p<0.01). what's more, every index of the mice in hige-dose Xiaoyaosan group were better significantly comparing with the ones in other groups (p<0.01).Conclusion: there were significantly the injury by oxidative stress and the changes of surviving condition of liver cell in CFS model mice. Xiaoyaosan can reduce the injury by oxidative stress, decreased the apoptosis of liver cells and decreased the damage of liver in CFS model mice. |
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