Correlation Between Neutrophil Gelatinase-associated Lipocalin(NGAL) And Preeclampsia(PE)

The definition of preeclampsia is the hypertension blood pressure appearing after 20 weeks of pregnancy and tend to normal 6 weeks postpartum [1]. Elevated blood pressure accompanied by proteinuria and edema are its characteristic. In recent years, the incidence has increased。It becomes an important reason of maternal/perinatal morbidity and mortality.It cannot be predicted or prevented accurately。The only effective treatment is delivery[2]. As such,early and accurate diagnosis is critical to optimize pregnancy outcome. Recent studies have shown that a number of biomarkers may be associated with preeclampsia,including several proangiogenic(VEGF, placental growth factor PLGF) and antiangiogenic factors(s FLT-1, s Eng)[3-14]. Neutrophil gelatinase-associated lipocalin(NGAL) is a newly discovered adipocyte factor, associated with oxidative stress [15]. Recent studies by D’Anna et al. investigated the use of serum NGAL as a pre-diagnostic marker of pre-eclampsia in each gestational trimester and reported higher serum NGAL concentrations in preeclamptic women compared to women with normotensive uncomplicated pregnancies, with significant differences in each trimester[16]. Objective:We detect the expression of NGAL in patients with different stages of hypertensive disorders in pregnancy, combined with the clinical features, to search the pathogenesis of preeclampsia, to explore the relationship between NGAL and preeclampsia, as well as the correlation between the level of serum NGAL and the the severity of PE,. For the prevention of clinical preeclampsia, early diagnosis andtimely treatment provide a good clinical indicators and prospects. Materials and methods:This paper selected a total of 80 cases of pregnant women treated in the Department of Obstetrics and Gynecology from July.2015 to Dec.2015 period, including 40 cases of preeclampsia, 13 patients with mild preeclampsia, 27 cases of severe pre-eclampsia. All groups were excepted for history of chronic hypertension, diabetes, chronic nephritis, and assisted reproductive birth defects and other factors. Another 40 cases of healthy pregnant women end in cesarean section due to head disproportion, breech presentation and other social factors. The maternal age, pre-pregnancy BMI, blood gestational age of the three team was not statistically significant(P> 0.05). Enzyme-linked immunosorbent assay(ELISA) were used to detect the serum NGAL levels in pregnant women and the cord blood of newborns in normal group, mild preeclampsia, severe preeclampsia.Diagnostic criteria for preeclampsia refer to "Obstetrics and Gynecology" 8th edition of the diagnostic criteria [1]. At the same time,we collect the clinical data: blood pressure at admission, random urine protein, liver and kidney function, 24-hour urine protein. Application SPSS19.0 statistical analysis. Results:1、Comparison of serum NGAL, between the preeclampsia group and the control group.Healthy control group, serum NGAL levels:( 8.39 ± 3.58) ng/m L, mild preeclampsia group, serum NGAL levels :( 14.13±3.72) ng/m L, severe preeclampsia group, serum NGAL levels:(23.17±9.59)ng/m L. The serum NGAL is significant different in the three groups(P<0.05);2、Comparison of the cord blood of newborns between the preeclampsia group and the control group.Healthy control group, fetal cord blood NGAL levels:( 6.15±3.42) ng/m L, mild preeclampsia group, fetal cord blood NGAL levels :( 15.42±4.33) ng/m L, severe preeclampsia group, fetal cord blood NGAL levels:(20.99±9.63)ng/m L. The serum NGAL is significant different in the three groups(P<0.05);3、Analysis of NGAL with the severity of diseaseAfter the detection of each pregnancy induced hypertension cases with NGAL in the blood, We conducted clinical features and statistical correlation analysis, the results are shown:Wherein the correlation coefficient systolic blood pressure was 0.438(p = 0.014), and diastolic blood pressure correlation coefficient was 0.488(p = 0.005), with a correlation coefficient of 24-hour urine total protein was 0.411(p=0.041). Conclusion:Without complication conditions1. The serum NGAL levels of Preeclampsia were increased than the normalcontrol group,which sever preeclampsia increases significantly.2. The NGAL in the cord blood of newborns levels of Preeclampsia wereincreased than the normal control group,which sever preeclampsiaincreases significantly.3. The serum NGAL levels of Preeclampsia has a positive correlation with theseverity of PE.