| Objective:Hypertensive disorder complicating pregnancy (HDCP) is one of the common complication of obstetrics, with the incidence of 9.4%～10.4% in China and 7%～12% abroad, is often the fatal disease that leads to the death of pregnant, parturient, fetus or neonate. Since its cause is obscure with complicated symptoms and limited treatments, it has long been the focus of the obstetrics research. With further development, more and more scholars have found Lipoprotein (a) and UA are closely related to the destruction of endodermis cells and they are the risky factors of cerebro-vascular disease; therefore, there is possibility that the two may be associated with the occurrence and development of hypertensive disorder complicating pregnancy. This paper discusses the relationship between the levels of the two in the patients of pre-eclampsia (mild, severe) and the severeness of disease by detecting the level of them in the blood samples collected from the maternal blood, placenta and cord blood of normal persons and patients with Pre-eclampsia.Methods: 90 cases for the caesarean patients hospitalizing in the SecondClinical Hospital of Jilin University from March 2008 to September 2009, with all the pregnant women having no complications in age and weeks (P>0.05). There are 30 normal cases, 20 cases in mild pre-eclampsia and 40 cases severe pre-eclampsia. Venus blood of 4mL from elbow will be taken from all the examiners after the abdomen is empty for 12 hours (before treatment for the hypertension patients), cord blood and placental blood are taken from caesarean, serum is separated within 30 min and then kept under -30℃. Immune turbidimetric analysis is adopted for Lipoprotein (a) and coupling colorimetric analysis for UA. Both are detected by means of Hitachi 7170 automatic biochemical analyzer.Statistical Methods:the results are analyzed by means of statistic software SPSS11.5,±s is used to express the amount, t or t ' is used to make a comparison between each group, P<0.05 refers to the significance of difference. Relevance and recurrence are used to denote the relations between the two variants.Results:(1) The relations between lipoprotein (a) and pre-eclampsia. ①levels of LP(a) in the maternal blood, cord blood and placental blood from mild PE group are all above the normal group(P<0.05); the levels of LP(a) in the maternal blood from severe PE group are all above the mild group and normal group(P<0.01); the levels of LP(a) in the cord blood and placental blood from mild PE group are all above the normal group(P<0.01); while it is of no significance in statistics compared with mild PE group(P>0.05).②levels of lipoprotein (a) in cord blood and placental blood (normal, mild severe PE group) is conspicuously lower than the maternal blood(P<0.01); while it is of no significance in statistics compared with levels of lipoprotein (a) in cord blood and placental blood(P>0.05). While the levels of lipoprotein (a) in cord blood of PE group (mild, severe) are lower than that of placental blood(P<0.05).(2) The relations between UA and pre-eclampsia.①levels of UA in the maternal blood, cord blood and placental blood from mild PE group are all above the normal group(P<0.05); the levels of UA in the maternal blood from severe PE group are all above the mild group and normal group(P<0.01).②it is of no significance in statistics compared with levels of UA among maternal blood, cord blood and placental blood(P>0.05).(3)Relations between lipoprotein (a) and UA of preeclampsia patients Severe pre-eclampsia: the relevance and recurrence analysis of lipoprotein (a) and UA, maternal blood: r =0.53, P=0.0004; blood of back placenta: r =0.41, P =0.0079 (this group may not be consistent with the normal distribution). The rise of level of lipoprotein (a) and UA is linear ( r =0.53) in the maternal blood of the severe pre-eclampsia,and the others are not, of no significance in statistics compared with mild PE group(P >0.05)。Conclusion:1. Lipoprotein (a) and UA can be treated as the reliable biochemical indicator in the development of pre-eclampsia.2. The severeness of pre-eclampsia is positively related to the lipoprotein (a) and UA, the level of lipoprotein (a) and UA is strikingly above that of normal gestation group and mild group(P<0.01).3. Lipoprotein (a) there is no striking difference of UA before and after placenta for the patients of pre-eclampsia, with the striking drop of lipoprotein (a), which may result from the placenta shield or the polymorphsism of the lipoprotein (a) structure. The evolusion of pre-eclampsia will lead to the change of lipoprotein (a) structure and lipoprotein (a ) can't pass the placenta shield, which needs to be further proved.4. The rise of level of lipoprotein (a) and UA is linear (the relative coefficient r =0.53) in the maternal blood of the severe pre-eclampsia. |
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