The Expression And Meaning Of R-spondin2, R-spondin4 And Dickkopf1 In Esophageal Cancer

Objective: As one of the commonest malignant tumors of digestive tract,Esophageal Cancer endangers human health severely and draws attention all over the world. China, with unknown pathogenesis, ranks the top in incidence and mortality of esophageal cancer in the world. Most people regard it as a complex pathological process of multiple gene changes and multiple stages of accumulation resulted from many factors such as biology, chemistry, and genetics, makes the study of pathogenesis as well as its control become an urgent problem to be solved.At present, the research on Wnt signal transduction pathway in Esophageal Cancer is more profound. Wnt mainly includes three signaling pathways, namely Wnt/β-catenin signaling pathway, Wnt/PCP(Planar Cell Polarity) signaling pathway and Wnt/Ca2+signaling pathway. Wnt/β-catenin signaling pathway is classical and the other two are non-classical ones.Researches are most commonly done on the classical one-Wnt/β-catenin signaling pathway. R-spondin(RSPO) is a newly discovered family in recent years. The main function of Wnt/β-caternin is to be an activator signal pathway, which enjoys great value on physiological and pathological research.However, the study of RSPO4 and RSPO2 in RSPO family has not been found in the study of Esophageal Cancer. Dickkopf1(DKK1) is another Antagonistic regulation factor in Wnt signal pathway, which shows abnormalities in many kinds of malignancies, but still rare in the study of Esophageal Cancer. This thesis detects 50 cases of Esophageal Cancer and the protein expression of RSPO2, RSPO4 and DKK1 of its adjacent normal tissues, and studies the relationship between it and the occurrence and development of Esophageal Cancer by using immunohistochemical SP method.1 Detect 50 cases of Esophageal Cancer and the protein expression of RSPO2, RSPO4 and DKK1 of its adjacent normal tissues by using immunohistochemical SP method.2 Statistical analysis of the data using statistical software SPSS13.0.Results:1 Expression of RSPO2 gene in Esophageal CancerIn 50 cases of Esophageal Cancer, the positive rate of protein expression of RSPO2 gene(66%,33/50) was significantly higher than that of adjacent normal tissues(30%,15/50), and the difference was with statistical significance(P=0.000). In Esophageal Cancer, the expression of RSPO2 gene was associated with the differentiation of tumor tissue(P=0.031) and staging of tumor(P=0.017), but not with age(P=0.903), sex(P=0.520) and lymph node metastasis(P=0.643) of patients.2 Expression of RSPO4 gene in Esophageal CancerIn 50 cases of Esophageal Cancer, the positive rate of protein expression of RSPO4 gene(64%,32/50) was significantly higher than that of adjacent normal tissues(34%,17/50), and the difference was with statistical significance(P=0.001). In Esophageal Cancer, the expression of RSPO4 gene was associated with the differentiation of tumor tissue(P=0.022) and staging of tumor(P=0.010), but not with age(P=0.797), sex(P=0.673) and lymph node metastasis(P=0.309) of patients.3 Expression of DKK1 gene in Esophageal CancerIn 50 cases of Esophageal Cancer, the positive rate of protein expression of DKK1 gene(70.0%,35/50) was significantly higher than that of adjacent normal tissues(28.0%,14/50), and the difference was with statistical significance(P=0.000). In Esophageal Cancer, the expression of DKK1 gene was associated with the differentiation of tumor tissue(P=0.017) and staging of tumor(P=0.008), but not with age(P=0.275), sex(P=0.515) and lymph node metastasis(P=0.577) of patients.Conclusions:1 The high expression of RSPO2,RSPO4 and DKK1 proteins in Methods:Esophageal Cancer may be related to the occurrence of esophageal squamous cell carcinoma.2 The expression of RSPO2, RSPO4 and DKK1 in Esophageal Cancer had nothing to do with sex, age and lymph node metastasis and was related to differentiation degree of tissue and the condition of clinical stages, etc. The expression of RSPO2, RSPO4 and DKK1 may be related to the malignant degree of Esophageal Cancer.3 The regulation of RSPO2, RSPO4 in the tumor is bidirectional, and it can provide a new direction for if it can provide targeted therapy for clinic and judgement prognosis.