The Research For Antioxidant Effective Parts Of NaoXueKang

Objective: Naoxuekang used in the clinical treatment of stroke show better results, stroke is ischemic cerebrovascular disease, can lead to ischemia-reperfusion injury, oxidative stress response. The study on Naoxuekang active antioxidant small molecules in vivo experiments, screening Naoxuekang having antioxidant activity and can be an effective component through the blood-brain barrier.Methods:1. The research of extraction Process for NaoxuekangIn the first factor DPPH antioxidant activity as an index optimize the extraction parameters. And two kinds of production before and after the optimization of process parameters obtained to compare Naoxuekang extract extract yield and DPPH activity.2. Separation and purification of small molecules peptides NaoxuekangNaoxuekang optimum extraction process to obtain a crude extract using ultrasonic disruption, as well as by low temperature extraction methods such as dextran gel column separated Naoxuekang small peptide component.3. Naoxuekang small peptides antioxidant ratingKunming mice as experimental subjects, treatment with orally administered small molecule peptide Naoxuekang components, determined by spectrophotometry mouse serum ROS content in antioxidant content of MDA and SOD activity evaluation Naoxuekang small molecules.4. Study Naoxuekang antioxidant BBB permeability of small peptidesThe use of cell membrane resistance measured Naoxuekang small molecular anti-brain barrier permeability component oxide group, to evaluate whether Naoxuekang antioxidant component can be used as treatment of brain oxidative stress injury of one kind of candidate drug group parts.5. Naoxuekang effective component relative molecular mass and antioxidant activity。By HPLC on Naoxuekang crude extract to determine the relative molecular mass distribution and the parts separated and purified after the group carried out; and in mouse serum SOD, MDA and ROS for the detection of indicators before and after the optimization process crude Naoxuekang after isolation and purification was mentioned components were compared antioxidant activity.Results: 1. The research of extraction Process for NaoxuekangExtraction process made in accordance with existing Naoxuekang extract extract yield 13.7%, free radical scavenging activity of DPPH antioxidant activity factor of 4.47; this experiment optimization of cerebral Naoxuekang extract obtained extract process yield 21.3%, free radical scavenging activity of DPPH antioxidant activity factor of 5.10.2. Separation and purification of small molecules peptides NaoxuekangThe use of sonication, low temperature extraction methods such Naoxuekang crude extract protein extraction, separation and further purified by dextran gel column chromatography, a yield of 0.3%3. Naoxuekang small peptides antioxidant ratingCompared with the control group, the treatment group Naoxuekang reduces ROS and MDA content and SOD activity increased, suggesting that the activity of small peptides Naoxuekang ROS scavenging and inhibition of MDA content increased the role and ability to eliminate oxygen free radicals.4. Study Naoxuekang antioxidant BBB permeability of small peptidesTransendothelial resistance value Naoxuekang experimental group had significant difference(P <0.01), showed Naoxuekang antioxidant small molecules peptides well through the blood brain barrier, it acts directly on the brain.5. Naoxuekang effective component relative molecular mass and antioxidant activityAfter separation and purification of small molecule component having an average molecular weight of 901.9, compared with the crude extract has stronger antioxidant activity.Conclusion: 1. Naoxuekang taken by cerebral process optimization to improve the extraction rate and enhance the antioxidant activity factor DPPH activity.2. Naoxuekang crude extracts after sonication and dextran gel column chromatography to obtain high purity small peptides component.3. Naoxuekang active small molecule ingredients with antioxidant effects.4. Naoxuekang extraction and isolation of small molecule components through the blood brain barrier.5. Small molecule ingredients by the separation and purification have high antioxidant effect.