Study On Resources And Quality Evaluation Of Tibetan Medicine Gyantse Seabuckthorn

Tibetan medicine gyantse seabuckthorn (Tibetan civilization, "Barr Dahl") is Elaeagnaceae plant dry ripe fruit of H. rhamnoides L. subsp. gyantsensis Rousi. Gyantsensis seabuckthorn was traditional tibetan medicinal materials traditionally used. "Crystal Pearl Materia Medica" records gyantse seabuckthorn has beneficial blood effects, but no experimental study reported. Therefore,cleared resources status, beneficial blood efficacy and chemical composition of gyantse seabuckthorn, established quality standards with a special property and fingerprints for gyantse seabuckthorn,It may provide a scientific basis for quality evaluation.Objective:1. The survey resource situation of gyantse seabuckthorn;2. Explore the impact of tibetan medicine Gyantse Seabuckthorn on blood stasis syndrome of the pattern of blood stasis syndrome of rats;3. Establish a specificity quality control method of gyantse seabuckthorn.Methods:1. Gyantse seabuckthorn samples combined with the reference literature review, field survey and visit the Tibetan Hospital manner seabuckthorn resources survey;2. Using the pattern of acute blood stasis syndrome of rats to study blood circulation efficacy of tibetan medicine Gyantse Seabuckthorn;3. The combination of literature and reference substance, using HPLC-MS technique to identify the structure of compounds in gyantse seabuckthorn;4. The method according to the 2010 edition of "Chinese Pharmacopoeia" A check items measured gyantse seabuckthorn moisture, total ash, acid insoluble ash and alcohol-soluble extract;5. Using polyamide TLC identify of Gyantse Seabuckthorn;6. Using HPLC method for the determination KSR,ISR,IGR,IR,IG five chemical composition of Gyantse seabuckthorn, and establish HPLC fingerprint of gyantse seabuckthorn.Results:1. Gyantse seabuckthorn resources are mainly distributed in the tibet autonomous region southeast valley, large reserves;2. Gyantse seabuckthorn on hemorheology acute blood abnormalities have significant improvement, its activity decreased with increasing dose;3. Combine literature, reference substance, and HPLC-MS analysis, Inferred QSR, KSR, ISR, IGR, IR, IG, KR 7 compounds of Gyantse seabuckthrn;4. Cleared character and established polyamide thin layer chromatographic identification of gyantse seabuckthorn. Measured the water, total ash, acid insoluble ash and alcohol-soluble extract content and KSR, ISR, IGR, IR, IG content of gyantse seabuckthorn herbs, calculated on dry goods, quality standards specified limits of not less than 0.050% KSR, IGR not less than 0.03%.5. Initially established HPLC fingerprint of gyantse seabuckthorn.Conclusion:1. Gyantse Seabuckthorn germplasm storage capacity is large, with a larger value for development;2. Tibetan medicine Gyantse seabuckthorn has a beneficial blood efficacy "crystal beads Materia Medica" recorded;3.Tibetan medicine gyantse seabuckthorn established quality standards and fingerprints.