| Objective:1. gestational hypertension animal model is set up.2. observation of salvia miltiorrhiza injection before and after the intervention the change of blood pressure and urine protein in rats.3. the soluble vascular endothelial growth factor receptor 1 (sFlt-1) and placental growth factor (PLGF) expression in rat placenta.4. analysis two kinds of factor expression content change in salvia miltiorrhiza injection before and after the intervention;This experiment aims to preliminarily salvia miltiorrhiza injection for the treatment of gestational hypertension model rats and the effect and the two factors and the relationship between gestational hypertension disease development, pathogenesis, diagnosis and treatment for clinical provide certain laboratory basis.Methods:1.36 female SD rats were randomly divided into 6 groups:normal control group, respectively (6), model group (6) and salvia miltiorrhiza dose in low dose group, danshen group (6) and salvia miltiorrhiza high-dose group (6), magnesium sulfate group (6).2. in the 13 weeks of pregnancy with N-nitro-L-arginine methyl ester (L NAME) gestational hypertension animal model is set up.3. on the 16th day daily intraperitoneal injection of low, medium and high dose of salvia miltiorrhiza injection, and the blank group, model group and magnesium sulfate intervention group by contrast, were observed before and after intervention in pregnant rats blood pressure and urine protein levels.4. in the 21 weeks of pregnancy, abdominal incision extraction, measuring the placenta weight, using reverse transcription-polymerase chain reaction (reverse transcription-polymerase chain reaction, rt-pcr) method to detect the contents of placenta sFIT-1, PLGF factor expression, so as to compare each two factors change of content of expression.Results:A. pregnant rats blood pressure valuesmodel group,10 days pregnant rats blood pressure is 101.34±12.54,15 days blood pressure was 124.81±13.40,19 days blood pressure was 127.69±12.07, comparing the two was statistically significant (P<0.05), said pregnant rats blood pressure rise before mould is made after the mould.15th day salvia miltiorrhiza, low dose group of pregnant rats blood pressure is 126.53 ± 13.25 mm,19 days blood pressure was 112.94 ± 9.51, salvia miltiorrhiza low dose group of 19 days blood pressure is conceived 15 days blood pressure lowering, but P 0.05, said two comparison there was no significant difference.3, salvia miltiorrhiza dose group of blood pressure was 125.64 ± 11.62,15 days pregnant at 19 days blood pressure was 109.64 ± 17.10, salvia miltiorrhiza high-dose group blood pressure was 125.25 ± 7.21,15 days pregnant at 19 days blood pressure was 106.94 ± 5.70, magnesium sulfate group blood pressure was 133.19 ±12.74,15 days pregnant at 19 days blood pressure was 108.11±3.08, comparing the two was statistically significant (P< 0.05), said in the salvia miltiorrhiza, high dose group, the magnesium sulfate group 19 days blood pressure is conceived 15 days to reduce blood pressure.B. the pregnant rat urine protein values1. the 10th day pregnant rats model groups but a value of 0.0064±0.0020,15 days blood pressure was 0.0131 ± 0.0074,19 days of pregnancy urine protein value of 0.0135 ±0.0014, comparing the two was statistically significant (P< 0.05), said pregnant rat urine protein value before making mould is made after the mould increases, and increases with the increase of days of pregnancy.2. salvia miltiorrhiza, low dose group of 15 days of pregnancy urine protein value of 0.0120±0.0026,19 days of pregnancy urine protein value of 0.0080 ± 0.0032, salvia miltiorrhiza dose group of 15 days of pregnancy urine protein value of 0.0120 ± 0.0027, 19 days of pregnancy urine protein value of 0.0077 ± 0.0018, salvia miltiorrhiza high dose group of 15 days of pregnancy urine protein value of 0.0112 ± 0.0029,19 days of pregnancy urine protein value of 0.0075 ± 0.0008,15 days of pregnancy magnesium sulfate groups value of 0.0123 ± 0.0025,19 days of pregnancy urine protein value of 0.0092 ± 0.0010. comparing the two was statistically significant (P<0.05); Said salvia miltiorrhiza low, medium and high dose group and magnesium sulfate group 19 days of pregnancy urine protein value less than 15 days of pregnancy urine protein.C. pregnant rat placenta sFIT-1 and the expression of PLGF factor, combined with the rt-pcr results, salvia miltiorrhiza low, medium and high dose group, the magnesium sulfate group sFIT-1 the expression of content is lower than the model group, comparing the two was statistically significant (P<0.05); Salvia miltiorrhiza low, medium and high dose group, the expression level of PLGF magnesium sulfate group were higher than that of model group, comparing the two was statistically significant (P<0.05).Conclusion:1. adopt the method of subcutaneous injection of L-NAME makes the building successful gestational hypertension rats.2. salvia miltiorrhiza injection in the legislation for activating blood circulation and lowers a model of pregnant rats blood pressure and urine protein levels.3. gestational hypertension model rat placenta the expression level of sFIT-1, salvia miltiorrhiza injection after the intervention, the expression level of sFIT-1.4. gestational hypertension model rats reduced the expression level of placental PLGF, salvia miltiorrhiza injection after the intervention, the expression level of PLGF rise. |
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