| Objective:1.To establish animal model for hypertensive disorder complicating pregnancy.2.To investigated the expression changes of blood pressure, urine protein and the average weight of fetal rats before and after the treatment of Astragalus injection.3.To investigated the expression changes of soluble vascular endothelial growth factor-1 receptor(sFlt-1) and placenta growth factor(PLGF) in rat placenta.4.To analyze the changes of the expression levels of two factors(sFlt-1 and PLGF) before and after the intervention of Astragalus injection, and to provide drug treatment data.Methods:1.36 female SD pregnant rats were randomly divided into for six groups:respectively as the blank control group(n = 6), model group(n = 6), low dose astragalus injection group(n = 6), Huangqi injection dose group(n = 6), high dose astragalus injection group(n = 6), magnesium sulfate in the control group(n = 6). On gestation 13 daily subcutaneous injection of nitro L arginine acid methyl ester(L-NAME) to establish pregnancy hypertensive rat model; in the 16 daily intraperitoneal injection of low, middle and high dosage of Astragalus injection, and the blank group, model group and magnesium sulfate group as control.2.Detection of blood pressure and 24 hours urinary protein in tenth, 15 and 19 days of pregnancy in 2 pregnant rats.3.On day 21 of gestation in caesarean section of the fetus, the measurement of fetal rat weight and reverse transcription polymerase chain reaction(reverse transcription polymerase chain reaction, reverse transcription polymerase chain reaction(RT-PCR) method for the detection of six groups of placenta of pregnant rats s FIT-1, PLGF expression and analysis of expression of the two factors in the content in each group.Results:1.Blood pressure in pregnant rats1.1 Model group, the pregnant mice were the 10 day average systolic blood pressure was 108.26 + 8.32 mm Hg, the 15 day average systolic blood pressure 131.75+ 689 mm Hg. The 19 day average systolic blood pressure to 134.87 + 8.33 mm Hg,pairwise comparison with statistical significance(P < 0.05) and expressed higher inpregnant rats average systolic blood pressure molding after than before modeling.1.2 Astragalus low dose groups of pregnant rats on the 15 th day average systolic blood pressure was 133.09 + 797 mm Hg, the 19 day average systolic blood pressure to127.74 + 13.81 mm Hg, Astragalus low dose group, the pregnant 19 days mean arterial pressure than the 15 th day of pregnancy mean arterial pressure decreased. However,P > 0.05, said pairwise comparison, there was no significant difference.1.3 Astragalus dose group pregnant for 15 days average systolic blood pressure was 133.01 + 840 mm Hg, pregnant for 19 days average systolic blood pressure was126.02 + 11.28 mm Hg. Highdose Astragalus group pregnant 15 days average systolic blood pressure was 129.07 + 3.42 mm Hg, pregnant for 19 days average systolic blood pressure was 11.298 + 9.94 mm Hg, magnesium sulfate group pregnant 15 days average systolic blood pressure 134.97 + 4.31 mm Hg, pregnant for 19 days average systolic blood pressure 112.47 + 12.50 mm Hg, pairwise comparison with statistical significance(P < 0.05), said the radix astragali, high dose group, the magnesium sulfate group, the pregnant 19 days average systolic blood pressure than the 15 th day of pregnancy average systolic blood pressure reduction.2.Urine protein of pregnant rats2.1 model group of pregnant rats on day 10 of the urine protein values was0.0064 + 0.0014mg/l. On the 15 th day of urine protein value 0.0119 + 0.0024,mg/l19 th day of pregnancy urine protein values 0.0172 + 0.0022mg/l, pairwise comparison with statistical significance(P < 0.05), indicating the pregnant rat urine protein values modeling than before modeling increased, and increased along with the increase of gestation days.2.2 Astragalus low dose group pregnant 15 days of urine protein values 0.0118 +0.0028mg/l, 19 of pregnancy urine protein values 0.0076 0.002mg/l, Astragalus dose group pregnant 15 days of urine protein values 0.0107 + 0.0028mg/l, 19 of pregnancy urine protein values 0.0077 + 0.0022mg/l, highdose Astragalus group pregnant 15 days of urine protein values 0.0113 + 0.0022mg/l, 19 of pregnancy urine protein values 0.0073 + 0.0025mg/l and magnesium sulfate group pregnant 15 days of urine protein values of 0.0127 + 0.0015mg/l, 19 of pregnancy urine protein values 0.0100 +0.0018mg/l, pairwise comparison was statistically significant(P < 0.05); Astragalus low, middle, high dose group and magnesium sulfate group 19 of pregnancy urine protein values below pregnant 15 days of urine protein values.3.The average weight of the rats in the low, middle and high dose group, the magnesium sulfate group, the average weight of the mice in the control group were higher than that in the model group, and the two two was statistically significant(P <0.05).4.Combined with RT-PCR expression analysis of placenta Pl GF and s FIT-1factor: low Astragalus, were significantly higher than those in model group, high dose group, the magnesium sulfate group, PLGF expression content, pairwise comparisons are statistically significant(P < 0.05); Astragalus low,, high dose group, the magnesium sulfate group s FIT-1 table of content of were lower than that of model group, pairwise comparison with statistical significance(P < 0.05).Conclusion:1.By giving pregnant rats by subcutaneous injection of L-NAME could successfully establish the rat model of hypertension of pregnancy.2 Astragalus injection can reduce the blood pressure and urine protein level, and increase the average weight of fetal rats.3.The expression of sFlt-1 in placenta of pregnancy induced hypertension model was more than that of normal pregnant rats, and the expression of PLGF in placenta of hypertensive rat model was lower than that in normal pregnant rats.4.The expression of sFlt-1 in the placenta of pregnancy induced hypertension model can be reduced, and the expression of PLGF in placenta of hypertensive rat model is increased after the injection of Astragalus injection. |
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