Fingerprint Spectra For Discrimination Of Rhizoma Panacis Japonici And Rhizoma Panacis Majoris

Rhizoma Panacis Japonici and Rhizoma Panacis Majoris were medicinal plants of Panax(Araliaceae), they were also the the valuable Chinese herbal medicine. Rhizoma Panacis Majoris was a variant of Rhizoma Panacis Japonici in plant sources. The close affinity between two kinds of medicinal materials led to the similar of chemical composition. With the deepening of the study of two kinds of medicinal material, it was of great significance that established chemical composition fingerprint spectra of them. This kind of research has not been reported. Based on the study of chemical composition, the chemical composition fingerprint spectra has been established with the methods of HPLC、UPLC-QTOF-MS/MS. The results of the study are as follows:HPLCanalysis:1. The fingerprint of Rhizoma Panax Japoncis and Rhizoma Panax Majors were established with the methed of HPLC. And all samples of Rhizoma Panacis Japonici and Rhizoma Panacis Majoris were analyzed. Furthermore, similarity calculation software, fuzzy poly-class method and the principal component analysis were used to differentiate and evaluate the established fingerprint spectra. Methodology of the study show that this method is reliable, accurate.2. Nineteen common peaks were defined in Rhizoma Panacis Japonici and Rhizoma Panacis Majoris herbs by fingerprint analysis for each other. Eleven of them were identified as ginsenoside Rg1, Re, Rb1, Rg2, Ro, Rd,Rg3, Rh2, Chikusetsu saponin IV, Ib, IVa by comparing with standard references or HPLC-MS/MS dates. Rhizoma Panacis Japonici and Rhizoma Panacis Majoris each have three peaks that can be separated with each other. The chromatography fingerprints similarity between Rhizoma Panacis Japonici and characteristic mode was0.892~0.992, the chromatography fingerprints similarity betweenRhizoma Panacis Majoris and characteristic mode was 0.899~0.950. The samples of Rhizoma Panacis Japonici was classfied into 3 clusters by principal component analysis and cluster analysis,the samples of Rhizoma Panacis Majoris was classfied into 2 clusters, and the all samples can be classfied into 2 clusters.UPLC-QTOF-MS/MS analysis:1. The samples were analyzed on the UPLC coupled with Q-TOF mass spectrometry. The UPLC-QTOF-MS/MS date of samples were subjected to principal component analysis in order to find the marker constituents. The samples of Rhizoma Panacis Japonici was classfied into 3 clusters,the marker constituents was ginsenoside Rg1,Chikusetsusaponin Ib, unknow peak(RT32.52 min、m/z 793.4356), the samples of Rhizoma Panacis Majoris was classfied into 2 clusters, the marker constituents was ginsenoside Rb1,Chikusetsusaponin IV, unknow peak(RT24.14 min、m/z 1087.5288).2. All samples was classfied into 2 clusters, the marker constituents was ginsenoside Rb1, ginsenoside Rf or pseudoGinsenoside F11, unknow peak(RT40.16min、m/z 793.4357), unknow peak(RT24.14 min、m/z 1087.5288). The result of PCA analysis is consistent with SPSS analysis. So these two herbs are able to distinguish by HPLC fingerprint spectra.3. Five kinds of Panax medicinal materials was analysed by the chemical composition fingerprint spectra. Some chromatographic peaks were identified.The result of chemical composition analysis and principal component analysis showed that five kinds of Panax medicinal materials has similarities as well as differences; the marker constituents was ginsenoside Ro,Chikusetsusaponin Ib、IV.The result showed that the newly chemical composition fingerprint spectra is useful for the discrimination of Panax medicinal materials.