The Evaluation Of Biocompatibility Of Iodine Modified TiO₂ Thin Film Coating PVC Tracheal Catheter

Experiment one Study of The cytotoxicity experiment iodine modified Ti O2 thin film coatings PVC endotracheal tubeObjective:To evaluate the cytotoxicity of the iodine modified Ti O2 film coated PVC endotracheal tube.Methods:According to the national standards of biological evaluation of medical devices, the preparation of the iodine modified Ti O2 film coated PVC tracheal tube extraction, using MTT colorimetric method; the experimental materials(iodine modified Ti O2 thin film coatings PVC endotracheal tube) extract, negative control(standard medical PVC endotracheal tube) leaching extract, positive control(dilute solution of phenol and saline) on mouse embryonic fibroblasts(L-929) were cultured in each group was calculated, the relative cell proliferation rate(P%) and microscopic observation of cell morphology, cell toxicity evaluation of experimental materials.Results:MTT results showed that the experimental material extract and the negative control group, cell toxicity was grade 1, the cells morphology under microscope, there was no obvious change, a normal spindle; positive control liquid cytotoxicity was grade 3, under the microscope, cells lose their normal shape, of the burr shape change. Conclusion:The iodine modified Ti O2 thin film coatings PVC endotracheal tube without obvious cytotoxicity,cell biocompatibility.Experiment two Stimulation of iodine modified Ti O2 thin film coatings PVC endotracheal tube skinObjective:To evaluate the iodine modified stimulation of Ti O2 thin film coating on the skin of the endotracheal tube is PVC.Methods:The drop of experimental material extract(iodine modified Ti O2 thin film coatings PVC tracheal catheter device extracts), negative control(normal saline solution(20%) and the positive control was twelve sodium dodecyl sulfate solution gauze dressing) respectively in 15 New Zealand albino rabbits skin hair removal area on both sides of the spine, closed bandage application of 6h, another research by not testing disposal information, records of 1h, 24 h, 48 h and 72 h reaction of the skin primary irritation score(PII), and calculated the average score is converted to the corresponding degree of reaction.Results:All animal experiments with 1h, 24 h, 48 h and 72 h in the removal of gauze close enough, the experimental material extracts and negative control contact site reactions, abnormal changes were not found, the type of reaction was very slight; positive control solution of the contact area with varying degrees of edema and erythema of the skin, the reaction type is moderate.Conclusion:The iodine modified Ti O2 thin film coatings PVC endotracheal tube without stimulation to the skin.Experiment three Delayed hypersensitivity test iodine modified Ti O2 thin film coatings PVC endotracheal tubeObjective:To evaluate the iodine modified Ti O2 thin film coatings PVC endotracheal tube on the skin sensitization.Methods:Repeated the soak test extracts and control extract liquid dressing gauze on guinea pig left back sticking, excitation induced by 24 h, 48 h, by another without the knowledge of the researchers according to Magnusson and Kligman grade reading, whether there is erythema and edema and allergic reaction in guinea pigs after being excited.Results:Abnormal situation does not appear during the experiment group and the control group experimental guinea pig, Magnusson and Kligman rating grade difference, the results were 0.Conclusion:The iodine modified Ti O2 thin film coatings PVC endotracheal tube for sensitization to skin.