The Influence Of Cisplatin And Cyclopamine On The Proliferation And Apoptosis In MG-63 Osteosarcoma Cells

Objective:To study the impact of cisplatin and cyclopamine, a Hedgehog signaling pathway inhibitor on the proliferation and apoptosis of MG-63 osteosarcoma cells.Methods:The proliferation of MG-63 cells was detected with the MTT assay and the apoptotic rate was analyzed with the flow cytometry assay.RT-PCR was used to detect the expressions of tumor-related genes in MG-63 cells before and after cyclopamine treatment.Results:The results show that cyclopamine inhibited the growth of MG-63 cells in time and dose dependent manners. After a 5,10 or 20μmol/L cyclopamine treatment for 48 hours,MG-63 cells showed increased apopotic rates significantly higher than those in the control group(p<0.05). After a 10μmol/L cyclopamine and a 2,4 or 8μmol/L cisplatin treatment for 24 hours,MG-63 cells showed increased apopotic rates higher than those in the control group and cyclopamine group(p < 0.05). Furthermore, cyclopamine down regulated the m RNA levels of PTCH1,Gli1 in MG-63 cells.Conclusion:Therefore, blockage of the Hedgehog signaling pathway with cyclopamine can suppress the proliferation and promote the apoptosis of MG-63 osteosarcoma cells.Cisplatin combined with cyclopamine can more effective inhibit the proliferation of osteosarcoma MG-63 cells and promote its apoptosis.