Examination Of Interferon (INF-γ) And Cisplatin (DDP) In Combination On Human Osteosarcoma Cell Line U-2OS Proliferation

ObjectiveObservation of interferon (INF-γ) and cisplatin (DDP) as monotherapy and in combination on human osteosarcoma cell line U-2OS proliferation.MethodsUsing MTT method (4-methyl thiazolyl tetrazolium assay) to detect cytotoxicity; The use of median-effect principle to determine the effect of drug combination; To observe the morphological changes of apoptosis with inverted phase contrast microscope; To analysis the effect of Cell cycle by flow cytometry.ResultsINF-γ,DDP alone on the human osteosarcoma cell line inhibited the growth of U-2OS, its role was positively correlated with the dose. Interferon in combination with cisplatin on osteosarcoma cell lines U-2OS proliferation and inhibition:interferon-γ(≤100u/ml) in combination with cisplatin for the antagonism on both (Q<0.85); interferon-y (≥1000u/ml) in combination with cisplatin for the additive effect (0.85