The Effect Of MiR-206 On The Expression Of Liver X Receptor-α In ApoE-KO Mice With Atherosclerosis

Objective:To investigate the effect of mi R-206 on the expression of liver X receptor-α in apo E-KO mice with atherosclerosis. Methods:30 apo E-KO mice aged 4 weeks old, fed with Western diet, were randomly divided as control group, mi R-206 mimic group and mi R-206 inhibitor group after 14 weeks. Every group contained 10 mice. Before sacrifice, the plasma was collected from tail vein to assay TC,TG,HDL-C and LDL-C. The changes of aortic atherosclerosis were observed by HE and red oil O staining, LXRα m RNA and protein levels were respectively determined by RT-PCR and Western blot. Peritoneal macrophages from apo E-KO mice were labeled with 3H-cholesterol and cholesterol efflux was detected. Results:The mi R-206 mimic group shows the increase of HDL compared with control gr oup. mi R-206 inhibitor group has higher TC and LDL levels than mi R-206 mimic and control group, but lower HDL level. After the HE and red oil O staining, there is a small plaque in the aortic lumen of control group, mi R-206 inhibitor group has the most severe pathological changes in the aortic lumen, while mi R-206 inhibitor group has little. To assess the cholesterol efflux show that the data of mi R-206 mimic group is higher than control gr oup, while the data of mi R-206 inhibitor group is lower. Conclusion:There may be some materials between mi R-206 and LXRα to constitute a feedback loop. As members of the self-regulated activity of LXRα, they all take part in the process of the reverse cholesterol transport.