Effects Of Ang(1-7) On The Expression Of Reverse Cholesterol Transport Related Factors In Atherosclerotic Rats

ObjectiveThrough the establishment of atherosclerotic rat models, investigate the effects of angiotensin(1-7)[Ang(1-7)] on the expression of reverse cholesterol transport related factors:High-density lipoprotein cholesterol (HDL-C)、ATP-binding cassette transporter Al(ABCA1)、Peroxisome proliferator-activated receptory(PPARγ)、 Liver X receptora(LXRα) and Retinoid X receptora(RXRa) in rats.Methods40healthy male SD rats were divided into normal control group, high-fat diet group, Ang(1-7) group and Ang(1-7)+A779group randomly. Continued to give Ang(1-7) by osmotic pump and jugular vein cannulation, after28d, HDL-C in rat plasma were detected,, gene expression of ABCA1、 PPARγ、 LXRa and RXRα in the aortic tissues of rats were measured by qRT-PCR and Western blot.ResultsCompared with the normal control group, HDL-C in high-fat diet group were significantly lower(P<0.01); compared with the high-fat diet group, HDL-C in Ang(1-7) group were significantly increased(P<0.01); compared with the Ang(1-7) group, HDL-C in Ang(1-7)+A779group were lower(P<0.05). Compared with the normal control group, ABCA1、 PPARγ、LXRα and RXRα mRNA and protein in high-fat diet group were significantly lower(P<0.01); compared with high-fat diet group, ABCA1、PPARγ、 LXRα and RXRα mRNA and protein in Ang(1-7) group were significantly increased(P<0.01); compared with Ang(1-7) group, ABCA1、 PPARγ、 LXRa and RXRa mRNA and protein in Ang(1-7)+A779group were lower(P<0.05). ConclusionAng(1-7) significantly raised HDL-C levels in atherosclerotic rat plasma, raised the gene expression of ABCA1、PPARγ、 LXRα and RXRα in the aortic tissues of rats, antagonizing the development of atherosclerosis.