| Objective: the effect to reduce the chemotherapy toxin of Malignant tumor and influence on immune function of Hui Sheng capsule.Methods: 170 Kunming inbred mice, half male and half female, vaccination sarcoma S-180 strain, randomly divided into four major groups according to sex and weight, each of 10 mice per group. The first large group to carry out anti-tumor experiments, divided into four little groups, each 10: tumor-bearing control group, C TX group, high-dose Hui Sheng capsule + CTX group, low-dose Hui Sheng capsules + C TX group. The second large group, to carry out C TX S-180 anti-tumor-bearing attenuated toxicity experiments, divided into five groups, each 10: normal control group, tumor-bearing control group, CTX group, high-dose Hui Sheng capsule + CTX group, low-dose Hui Sheng capsules + CTX group. The third large group for serum hemolysin content experiments, the fourth large group for tumor-bearing mouse spleen lymphocyte proliferation index experiments, both third and forth large group divided into four little groups, each 10: normal control group, tumor-bearing control group, high-dose Hui Sheng capsule group, low-dose Hui Sheng capsule group. S-180 tumor-bearing mice by mouth resuscitation Hui Sheng capsule for 10 days, the tumor weighed to calculate the inhibition rate and measuring the average tumor weight; simultaneous determination of red blood cell(RBC), white blood cell(WBC), platelet count(PLT). Calculated by MTT staining lymphocyte proliferation index, HC50 hemolysin concentration determination. 170 Kunming inbred mice, half male and half female, vaccination sarcoma S-180 strain, randomly divided into four major groups according to sex and weight, each of 10 mice per group. The first large group to carry out anti-tumor experiments, divided into four little groups, each 10: tumor-bearing control group, CTX group, high-dose Hui Sheng capsule + C TX group, low-dose Hui Sheng capsules + CTX group. The second large group, to carry out CTX S-180 anti-tumor-bearing attenuated toxicity experiments, divided into five groups, each 10: normal control group, tumor-bearing control group, CTX group, high-dose Hui Sheng capsule + C TX group, low-dose Hui Sheng capsules + CTX group. The third large group for serum hemolysin content experiments, the fourth large group for tumor-bearing mouse spleen lymphocyte proliferation index experime nts, both third and forth large group divided into four little groups, each 10: normal control group, tumor-bearing control group, high-dose Hui Sheng capsule group, low-dose Hui Sheng capsule group. S-180 tumor-bearing mice by mouth resuscitation Hui Sheng capsule for 10 days, the tumor weighed to calculate the inhibition rate and measuring the average tumor weight; simultaneous determination of red blood cell(RBC), white blood cell(WBC), platelet count(PLT). Calculated by MTT staining lymphocyte proliferation index, HC50 hemolysin concentration determination.(1) Inhibitory effect in tumor-bearing S-180 mice: C TX can reduce the wight of S-180 sarcoma in S-180-sarcoma mice, comparing tumor-bearing control group and C TX group, there was a significant difference(P<0.01). Combine Hui Sheng capsules and C TX, can also reduce the weight of S-180 sarcoma, the tumor inhibitory rate of high-dose Hui Sheng capsule group was 49.4%, the tumor inhibitory rate of small-dose Hui Sheng capsule group was 48.8%, both significa ntly different with CTX group(P <0.05).(2)The attenuation effect of C TX against S-180 sarcoma toxicity: tumor-bearing control group mice’s RBC, WBC and PLT no significant change, comparing with the normal control group, there’s no significant difference(P>0.05). CTX can reduce tumor-bearing mice’s RBC and WBC, comparing normal control group with CTX group, there was a extremely significant difference(P<0.01) and for tumor-bearing control group,there was a significant difference(P <0.05), but the tumor-bearing mice’s PLT had no significant change. Hui Sheng capsules and CTX used in combination, can against CTX reduced RBC and WBC of tumor-bearing mice, compared with the CTX group, high-dose group had a significant difference(P <0.01).(3)Effect of splenic lymphocyte proliferation in tumor-bearing mice: tumor-bearing control group mice’s lymphocyte proliferation index decreased, between tumor-bearing control group and the control group, there was a significant difference(P<0.01). Hui Sheng capsules can increase the lymphocyte proliferation index, compared with tumor-bearing control group, Hui Sheng capsule large and small dose group showed significant differences(P <0.05).(4) Effect of Hui Sheng capsule hemolysin content on tumor-bearing mice: tumor-bearing control group mice have a reduced level of serum hemolysin, comparing normal control group and tumor-bearing control group, there was a significant difference(P<0.01). Hui Sheng capsule can improve the level of serum hemolysin in mice, compared with the tumor-bearing control group, large-dose Hui Sheng capsule has extremely significant differcance and small-dose group has significant difference(P <0.05).Conclusion: the Hui Sheng capsule has obvious inhibition on the S-180 tumor-burdened mice tumor, relieve s the side effect of toxin, improves the life quality of cancer patients and reduces the side effects, which bases on the mechanism to adjust the immune function in tumor-burdened mice, inhibit the proliferation of tumor cell for the purpose of treatment. |
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