The Accuracy Of Neutrophil CD64 Diagnosing Bacterial Infection In Patients With Hematologic Malignancies After Chemotherapy

Objective:This study was aimed to investigate the value of CD64 mean fluorescence intensity on the peripheral blood neutrophils as a diagnostic marker of bacterial infection in patients with hematologic malignancies after chemotherapy. It could provide a reference for early clinical diagnosis of bacterial infection and avoid the abuse of antibiotics. Methods:We choose 196 blood cancer patients who were getting standard chemotherapy in the second affiliated hospital of nanchang university from June 2013 to June 2014, including 121 cases of leukemia, 34 cases of lymphoma, 41 cases of multiple myeloma, among which 116 were male, 80 were female, ages 18 to 78 years, the average(40.37±16.35). 1. According to the patient’s signs and symptoms of infection, biochemical and imaging examination results, we can have a comprehensive analysis of all the object of study, these patients could be divided into the infected group and uninfected group. Neutrophil CD64 mean fluorescence intensity of all patients was detected by flow cytometry, and was compared with procalcitonin(PCT) and C reactive protein(CRP) detected in part of patients. 2. Through continuous dynamic monitoring patients with no fever and whose hospitalization period was longer, to collect peripheral blood test its nCD64 mean fluorescence intensity once every 2 days. To analysize the relationship between nCD64 mean fluorescence intensity and bacterial infection. Test results were analyzed by using SPSS19.0 statistical software, and the measurement data used mean±standard deviation to express. Comparing with the sensitivity and specificity of nCD64 mean fluorescence intensity and PCT and CRP in diagnosis of bacterial infection in patients with hematologic malignancies. Using ROC curve to show statistical processing, P < 0.01 was considered statistically significant. Results:1. The expression of nCD64 is not affected by the number of neutrophils(P > 0.01); The nCD64 mean fluorescence intensity, PCT and CRP in infection group and dynamic monitoring group were significantly higher than that in non-infected group(P < 0.01); 2. nCD64 mean fluorescence intensity is possessed of much higher in the sensitivity and specificity, compared with PCT and CRP in diagnosis of bacterial infection. Conclusion:nCD64 mean fluorescence intensity can be used as an effective diagnostic marker of bacterial infection in patients with hematologic malignancies after chemotherapy. To some extent, it can forecast bacterial infection. Clinician can use of antibiotics timely and effectively, and can reduce the risk of patients with chemotherapy.