M-FISH In Detecting Complex Chromosomal Aberrations In Hematologic Malignancies

Objective To explore the value of the technique of multiplex fluorescence in situ hybridization (M-FISH) in the detection of the complex chromosomal aberrations (CCAs) of hematologic malignancies.Methods M-FISH was used in 5 acute lymphoblastic leukemia (ALL) patients, 10 myelodysplastic syndromes (MDS) patients and 5 multiple myeloma (MM) patients with CCAs detected by R-banding to refine complex chromosomal rearrangements, the constitute of marker chromosomes, and to identify cryptic translocations.Results At first we established the technic system of M-FISH by detecting 1 male patient with normal karyotype and 5 ALL patients with CCAs. In MDS, 37 kinds of structural rearrangements were detected by M-FISH including insertion, deletion, translocation and derivative chromosomes, and among them 34 kinds were unbalanced rearrangements, only 3 were balanced rearrangements including t(6;22)(q21;ql2), t(9;19)(ql3;pl3) and t(3;5)(?;?), 7 abnormalities were...