Association Study On Relationship Between Genetic Polymorphisms With The Reabsorption Of Uric Acid And Hyperuricemia

Objective To study the relationship between genetic polymorphisms with the reabsorption of uric acid and the incidence of hyperuricemia in Ningxia.To provide a theoretical basis for the study on population genetics of Hui and Han population.Method 1. According to a 1:1 matched case-control study, selected 370 unrelated hyperuricemia patients aged from 29 to 80 years old in a medical institution in the Ningxia Hui Autonomous Region. Matched by gender, age and ethnicity, 370 unrelated healthy population aged from 28 to 81 years old who have a regular health examination in the same location and period were selected as a control group. There were 200 Hui and 170 Han in the 231 men and 139 women in both groups, mean age(6.84 ±9.37) years old in the case group and mean age(50.38±6.78) years old in the control group.2. We used the self-designed questionnaire to survey the general, lifestyles, family histories and medical histories, did the physical measurements and laboratory biochemical blood tests. SLC2A9 gene rs13129697 and rs1014290 sites and SLC22A12 gene rs7932775 locus were detected by Sequenom Mass ARRAY technology.Paried T test was used to compare general clinical data and biochemical indexes of the two groups. By χ2 test, we compared the frequencies of the genotype and allele in each group. Logistic regression method was used to analyze the hyperuricemia affected by gene polymorphism and general information. Multiple linear regression analysis was applied to find the relationship between genotype, general information and uric acid levels.Results 1. The levels of SUA, Crea, SBP, TC and BMI in the patients were higher than those in the control group(all P<0.05). The BMI, SBP, SUA, Crea, TC and TG in male patients were higher than those in control group, and the Crea, TG and SUA in female patients were higher than those in control group(all P<0.05).2. The differences of SBP, SUA, crea, TG were statistical significance between Han male cases and control group. The differences of SUA, crea, TG were statistical significance between Han male cases and control group(all P<0.05). The differences of SUA, crea, BMI, FPG, HDL, TG, SBP, DBP between different genders, were statistically significant between Han case group(all P<0.05); The differences of SUA, crea, TC, H-LDL, L-LDL, BMI, SBP, DBP differences between different genders were statistically significant in Han control group(all P<0.05).3. The differences of BMI, SUA, crea, TC, TG differences were statistical significance between Hui male cases and control group(all P<0.05).The difference of SUA and crea has statistical significance between Hui female patient group and control group(all P<0.05). The difference of BMI, crea, SUA between different genders were statistically significant in Hui case group(all P<0.05).The difference of BMI, SUA between different genders were statistically significant in Hui control group(all P<0.05).4. There were significant differences in the distribution of G/G, G/A, A/A genotypes and A, G allele frequencies of SLC2A9 gene rs1014290 between the hyperuricemia group and control group(all P<0.05). A/A, G/A and G/A+A/A genotypes carriers increase the risk of hyperuricemia(P=0.004,OR=2.569,95%CI:1.361~4.848;P=0.001,OR=3.375,95%CI:1.599~7.126;P=0.000,OR=2.181,95%CI:1.411~3.372).There were significant differences in the distribution of genotypes and allele frequencies of between the HUA group and control group in Hui and Han people(all P<0.05).5. There were no differences in the distribution of T/T, G/T, G/G genotypes frequencies of SLC2A9 gene rs13129697 between the hyperuricemia group and control group(P>0.05). There were significant differences in distribution the of T, G allele frequencies between the hyperuricemia group and control group(P<0.05). G/T+T/T genotypes carriers increase the risk of hyperuricemia(P=0.045, OR=1.456, 95%CI: 1.008~2.103).There were significant differences in the distribution of allele frequencies of between the hyperuricemia group and control group in Hui people(P<0.05). There were no differences in the distribution of genotypes and allele frequencies between the hyperuricemia group and control group(all P>0.05).6. There were significant differences in distribution the of C/C, C/T,T/T genotypes frequencies between the hyperuricemia group and control group(P<0.05). There were no differences in the distribution of C,T allele frequencies of SLC22A12 gene rs7932775 between the hyperuricemia group and control group(P > 0.05).There were significant differences in the distribution of allele frequencies of genotypes frequencies between the hyperuricemia group and control group in Hui people(all P<0.05). There were no differences in the distribution of genotypes frequencies between the hyperuricemia group and control group in Han people(P>0.05). There were significant differences in the distribution of allele frequencies of genotypes between the two groups in Han paople(P<0.05).7. Logistic regression analysis showed that BMI, TG, HDL, Crea, female, Hui nationality,SLC2A9 gene rs1014290(G/A and A/A genotype) were risk factors for hyperuricemia(P<0.05). SBP、Crea、gene rs1014290(G/A and A/A genotype) were risk factors for hyperuricemia in Hui people and BMI、Crea、TG、female were risk factors for hyperuricemia in Han people(P<0.05). BMI, Crea, TG, Hui people, rs1014290(G/A and A/A genotype) were risk factors for male and Crea was risk factor for female(P<0.05).8. Multiple linear regression analysis showed that Crea, BMI, Gender, Nationality and LDL were related to SUA level. Gender, Crea, BMI and TC entered the regression model in the case group. Crea, Gender, TG, DBP and Age entered the regression model in the control group.Conclusion:1. Polymorphisms of SLC2A9 gene rs1014290, rs13129697 and SLC22A12 gene rs7932775 were significant correlation with hyperuricemia between Hui and Han people in Ningxia.2. In this study, it was probably that SLC2A9 gene rs1014290 A allele, rs13129697 T allele and SLC22A12 gene rs7932775 T allele carriers increase the risk of hyperuricemia.3. It was probably that the factors of hyperuricemia included Female, BMI, TG, HDL, Hui nationality, Crea and SLC2A9 gene rs1014290(G/A and A/A genotype).