Studies On The Chemical Constituents And Hypoglycemic Activity Of Cicer Arietinum

This paper studied on chemical composition, determining isoflavones by the emendation method of ultraviolet-visible spectrophotometry, screening for hypoglycemic activity of different extraction parts,mechanism of action,structure modification of genistein,cell hypoglycemic activity of ethoxylate products and genistein of the seeds of Cicer arietinum L.On chemistry, seven compounds were separated from the different extraction parts by using silica gel column chromatography, gel column chromatography(Sephadex LH-20),high-speed countercurrent chromatography, high performance liquid chromatography from the seeds of Cicer arietinum L. and identified by 1H-NMR,13C-NMR,DEPT,ESI and the five compounds were β-sitosterol, ferulic acid, biochanin A, genistein, diisobutyl phthalate.The ferulic acid was the first time isolated from the seeds of Cicer arietinum L.The basic parameter settings of the separation of ethyl acetate parts and 95% ethanol parts :flow rate of stationary phase 2ml/min, flow rate of mobile phase 0.5ml/min rotational speed of the main engine 1800r/min, run clockwise,temperature25℃,the separation of ethyl acetate parts of the best solvent system: normal hexane-ethyl acetate-methanol-water(6:4:4.2:1, v/v/v/v),wavelength 254 nm,stationary phase retention 65%.the separation of 95% ethyl alcohol part of the best solvent system: chloroform-methanol-water(4:2.5:2, v/v/v),wavelength 254 nm,stationary phase retention 65%.It is simple and fast to use ultraviolet spectrophotometry measuring total isoflavones content, but the determination results are often much bigger when using a standard product measuring a variety of isoflavones composition. This study improved the accuracy of the method using correction method. The average recovery before correction is 93.74% and the average recovery after correction is 97.95%.The average recovery after correction increased 4.25% than the average recovery before correction. With the increase of mixed standard liquid, the recovery of the difference degree is becoming bigger, the improvement of measurement accuracy is more obvious.The seeds of Cicer arietinum L. were extracted by using diacolation with different polarity solvent. Petroleum ether part, ethyl acetate part, 95% ethanol part, 70% ethanol part and 30% ethanol part were got respectively. Congenital diabetic KKAy mice were used to select the best hypoglycemic active part. Determination of five different parts have different effects on the body weight, the quantity of diet,the quantity of drinking water,fasting blood glucose,insulin tolerance test,TG,TC,HDL,LDL,Kidney body ratio,Liver body ratio in mice, ethyl acetate part as the most obvious active part has the best hypoglycemic effect. The affection of ethyl acetate part was explored for PPAR α and PPAR γ. The results showed that ethyl acetate part has the largest influence on each index.Ethyl acetate part maybe regulate PPAR α and PPAR γ gene expression to improve the insulin resistance directly or indirectly, thereby playing reducing blood glucose, treating diabetes role.Genistein as one of isoflavones, have the function of female hormone, anti-oxidation, reducing blood fat, increase bone densit. Genistein fat-soluble and water-soluble are poor. 5,7,4’-triethoxy genistein and 7,4’-2 ethoxy genistein were synthesized by ethoxylation structure modification of Genistein. Genistein fat-soluble was increased. Genistein and two compounds were used to do the experimental of cell hypoglycemic activity. The results showed that 5,7,4’-triethoxy has better hypoglycemic activity in 92.5μmol/L,compared with genistein, statistically significant difference(P<0.05).7,4’-2 ethoxy genistein has better hypoglycemic activity in 277.5μmol/L,compared with genistein, statistically significant difference(P < 0.05).