Mechanism Study On Blood-sugar-lowering And Lipid-adjusting Of Total Saponine In Cicer Arietinum

Objective:To observe the effects of Cicerarinum extract and Total Saponins of Cicer Arietinum (TSCA Ⅰ and TSCAⅡ) onglucose lipid metabolism of type2diabetes rats and to discuss the mechanism from different aspect preliminarily.Methods:Animal model of type2diabeteswas established by a high fat dietwith intraperitoneal injection of streptozocin (low dose) into male SD rats separated to10groups:controlgroup, modelgroup, metformingroup, Lovastatingroup low-dose (0.7g·Kg-1), middle-dose (1.4g·Kg-1), high-dose (2.8g·Kg-1) of compound A and low-dose (0.8g·Kg-1), middle-dose (1.6g·Kg-1), high-dose (3.2g·Kg-1) of compound B of Cicerari-numl extract. After4weeks treatment (once a day), weigh them. The level of fasting plasmaglucose (FPG), fasting insulin (FIns), total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), insulin sensitivity index (ISI) and the pathological changes of the liver and kidneywere evaluated.Animal model of type2diabeteswas established andgive controlgroup, modelgroup, metformingroup (0.1g·Kg-1), low-dose (0.1g·Kg-1), high-dose (0.3g·Kg-1) of Cicer Arietinum Total Saponins (TSCA Ⅰ and TSCA Ⅱ). After4weeks treatment (one time a day), weight them. The level of FPG, FIns, TC, TG, HDL-C, LDL-C, insulin resistance index (IRI), free fatty acid (FFA), interleukin (IL-6), muscleglycogen/hepaticglycogen (MG/HG), hexokinase (HK) in muscle and the pathological changes of the liver and kidneywere evaluated ISI and the pathological changes of the pancreaswere evaluated. Serum creatinine (Scr), blood urea nitrogen (BUN), angiotensin Ⅱ(AT Ⅱ), endothelin-1 (ET-1) content, plasma thromboxane B (TXB2) and6-Keto-PGF1α in the content, to examine the impact of TSCA substances of the blood vessels; total superoxide dismutase (TSOD), catalase (CAT), malondialdehyde (MDA), total nitric oxide enzyme (TNOS) activity detection, inspection TSCA antioxidant activity; detect the level of PPAR-ygene transcription.Results:Comparedwith modelgroup, the liver index in Cicerarinuml extractgroup declined, the level of FPG declined and ISI increased-except low-dosegroup of compound B, the level of TC of high-dosegroup of compound A declined, the level of LDL-C of high-dosegroup of compound B declined, and the levels of TG, TC, LDL-C of all others Cicerarinuml extractgroup declined. Cicerarinuml extractgroup can lighten the pathological lesion of the liver and kidney.Comparedwith the modelgroup, the level of FPG, TC, TG, LDL-C, FIns, IRI, FFA of blood serum and IL-6were reduced significantly by treatedwith TSCA Ⅰ and TSCAⅡ (P <0.05, P<0.01), however the HDL-C/TC, MG, HG and the activity of hexokinasewere elevatated significantly (P<0.05, P<0.01), injured pancreatic isletwere regulated aswell. And the TSCA Ⅱ had better effect than TSCA Ⅰ in decreasing hyperglycemia. TSCA Ⅱ to reduce diabetic rats TXB2and6-Keto-PGF1α in and other vascular substances, renal vascular repair; the TSCAⅡ can improve the antioxidant capacity of type2diabetic rats, and its main active site is the liver, kidney andskeletal muscle; followed by TSCAⅡ increase in type2diabetic rats PPAR-ygene transcription level.Conclusions:Cicerarinuml extract and TSCA can adjust the bloodglucose and lipid effectively. The possible mechanism is considered to reduce the level of FFA and IL-6in order to ameliorate IR and elevatate the activity of hexokinase andglycogen, Repair of pancreatic islets and renal blood vessels, increase the antioxidant capacity and increase PPAR-ygene transcription level.