Microbial Transformation Of Ginsenosides And Protective Effect Of Ginsenoside Compand K Against Cisplatin-induced Acute Kidney Injury In Mice

In this study, four microbial strains were selected,Bacillus coagulans, Escherichia coli, Lactic acid bacteria and yeast, to ferment White ginseng.The content of total saponins after fermentation were detected by UV Spectrophotometric Determination. The results showed that total saponins contents of the fermented White ginseng ranged from 3.24% to 5.21%. However, the total saponins contents of the White ginseng fermented by Bacillus coagulans increased about 31%, which were the most significantly elevated levels.Next, the content of nine kinds ginsenosides after fermentated in Ginsenoside of stems and leaves(GSL) and White ginseng were detected respectively by HPLC. The chromatographic conditions as follows,Agilent Eclipse XDB-C18(250mm×4.6mm, 5μm),mobile phase :acetonitrile-water gradient elution,detection wavelength 203 nm,and the flow rate:1.0mL/min,the injection volume is 10μl. The results show that,after fermentation in contents of ginsenoside saponins in White ginseng and GSL showed some changes, However, the contents of the ginsenoside Escherichia coli, Lactic acid bacteria and Yeast were not significantly, but the content of ginsenoside Rd and Rb3 decreased significantly after fermented by Bacillus coagulans, that means the generation of the rare ginsenoside. By testing the TLC spot color and size determinated Bacillus coagulans can be transformed raw ginseng to Ginsenoside CK,and indeed confirmed as Ginsenoside CK by HPLC. Chromatographic conditions were XDB-C18(4.6mm×250mm, 5μm), the mobile phase of acetonitrile: water =45:55, the column temperature was 30℃, the detection wavelength was 203 nm, and the flow rate was 1mL/min.In this paper, on the basis of single factor experiment, the optimization of fermentation conditions of Bacillus coagulans was established with orthogonal design, contect of Ginsenoside CK was used as the indicator,the optimum conditions were showed as: the culture temperature was 35℃, inoculum was 1%, revolution of cradle was 160 r /min, fermentation time was 36 h. Then, the fermentation product was purified by silica gel column chromatography,the purified was identificate as Ginsenoside CK by the modern spectral analysis methods,HPCL and NMR detection,it’s purity was more than 90%.This papert established a model of acute kidney injury in mice,to observed renal protective effect of ginsenoside CK in mice. Mice were randomly divided into control group, model group, ginsenoside CK low dose(20mg/kg), ginsenoside CK high dose(40mg/kg). Ginsenoside CK continuous administration 10 d, body weight was measured daily and food and fluid intake were monitored every day. Finally, the mice were sacrificed to measured blood serum BUN, Scr content, and renal tissues weighed kidney index calculation. Simultaneous determined the SOD,GSH and MDA activity of kidney tissue. The experimental results show that ginsenosides CK 20,40mg/kg dose group could improve mouse kidney tissue morphology, inhibiting the activity of SOD and GSH decreased, decreasing MDA content in kidney tissue, while reducing serum levels of BUN and Scr. It means that ginsenoside CK have a protective effect on cisplatin-induced acute kidney injury of mice. Ginsenosides CK 20mg/kg and 40mg/kg dose group showed a significant difference, of which effect the 20mg/kg dose group was better.