| Objective:To objective the effects of two different concentrations of HTS in fluid resuscitation of uncontrolled hemorrhagic shock rabbits.Methods:40 rabbits were divided into five groups randomly, they were SHAM group, SWT group, NS group, 4.5% group and 7.5% group. Established the uncontrolled hemorrhagic shock model in rabbits, and then waited 15 minutes. Every group used a predetermined scheme fluid resuscitation when the rabbits were stable. Monitored following dates at the experimental time of 0min, 30 min, 60 min and 90min:1. Hemodynamics(LVSP, ± dp/ dtmax, CVP, HR), monitored by the BL-420 F.2. Serum Cr and serum BUN, blood samples were taken at 0min, 30 min, 60 min and90min, 3000 rpm centrifuged 15 min, kept the serum, detect serum BUN and serum Cr with BUN and Cr kits.3. At the end of the experiment, statisticed the volume of bleeding and transfusion,heart, lung and kidney tissue sections stained with HE.Results:1. The age, baseline weight and initial MAP of rabbits in each group were analyzed by ANOVA, and no significant difference(P> 0.05).2. The effects of acute blood loss operation on rabbit hemodynamic and renal function.After acute blood loss operation, LVSP, ± dp/dtmax, and CVP of SWT group, NS group, 4.5% and 7.5% were significantly decreased, and arrived lowest at 15min; at 30 min,the LVSP, ±dp/dtmax and CVP of the four groups had no statistically significant difference through pairwise comparison. Serum Cr and serum BUN were significantly increased, and had no statistically significant through pairwise comparison but were significantly higher than SHAM Group, HR did not change significantly during the experiment.3. The effects of fluid resuscitation on rabbit hemodynamic and renal function.After giving fluid resuscitation, LVSP, ± dp/dtmax and CVP of NS Group, 4.5% Group,7.5% Group increased at varying degrees, and at 45 min reached a peak, compared with the SWT Group, the differences had statistically significant(P<0.05); BUN and Cr of the three groups has decreased after resuscitation, and were significantly lower than that of the SWT Group(P<0.05).The improvement of LVSP, ±dp/dtmax of 7.5% Group and 4.5% Group is significantly higher than NS Group, and 7.5% Group is also significantly higher than 4.5%group(P<0.05). BUN, Cr of 7.5% Group is significantly lower than NS Group(P<0.05).There was no statistical significance difference in CVP between the three groups(P> 0.05).Fluid infusion volume of each period and the total in 4.5% Group and 7.5% Group had no statistically significant difference, but the total fluid infusion volume of 7.5% Group is significantly lower than NS Group(P<0.05). Fluid infusion can increase the continuous bleeding, and NS Group is the highest, 7.5% Group is the lowest, but the difference was not statistically significant between the three groups.4. Comparision of the infusion volume and continue bleeding.The infusion volume of NS Group, 4.5% Group, 7.5% Group in different time period had no significant difference, but the total infusion volume of 7.5% Group wassignificantly lower than the NS Group(NS Group=72.13±9.46 ml, 4.5% Group=57.00±15.49 ml, 7.5% Group=52.00±17.81ml). After infusion, the continue bleeding of NS Group,4.5% Group, 7.5% Group was increased, and the NS Group was the highest(6.15±0.56ml),the 7.5% Group was the lowest(4.89±0.34ml), but there was no significant difference between the three groups.5. HE staining results.Pathology of renal, heart and lung tissue in SHAM Group were normal, organ damage was serious in SWT Group, pathological changed in NS Group, 4.5% Group and7.5% Groups, but the changes was slightly lighter than the SWT Group, and there were not significantly different in organ pathological changes between the three groups.Conclusion:1. The rabbit model of UHS successfully established by drawing blood from femoral artery, and this model can simulate the state of wounded.2. Fluid resuscitation can improve the hemodynamic and renal function of UHS rabbits.3. The effects of HTS is better than NS.4. The effects of 7.5% HTS in treatment of UHS rabbits were better than 4.5% HTS. |
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