Effect Of Different Sodium Concentration Of Hypertonic Saline Resuscitation On Hepatic Injury In Severely Scalded Rats

Objectives To explore the effect of different sodium concentration of hypertonic saline(600,800,1000mmol/LNa~+)resuscitation on hepatic injury in severely scalded rats versus lactated Ringer solution(LR)though hepatic cell morpholgy,signal transduction pathway,inflammatory cytokines,oxidative damage.Materials and methods 104 SD female rats,weighed 200g-250 g,were randomly divided into 5 groups: sham-burn group,sodium lactate Ringer's solution group(LR group)and 3 hypertonic sodium solution groups(600,800,1000 HS group).All rats were catheterizated by tail vein after anesthetization,and burn resuscitation therapy was useed in the rats of burned groups as soon as they were given the 30% burn trauma.The abdominal aortic blood and liver tissue were acquisited in the sham-burn group immediately and LR and HS groups at all times post trauma.Part ? : The effect of different sodium concentration of hypertonic saline resuscitation on hepatic dysfunction and cellular damge in severely scalded rats.Take out a part of liver tissue and plasma from five sets of rats.Automatic biochemical analyzers were used to detect the serum ALT and AST levels.The fixed liver tissue using 10% formaldehyde were used to observe the tissue cell morphology by HE staining methodPart ?: The effect of different sodium concentration of hypertonic saline resuscitation on oxidative damage and inflammatory factors in severely scalded rats.Take out a part of liver tissue and plasma from five sets of rats,Colorimetric method was used on determination about the MDA content and SOD activity of liver tissue.ELISA was used in the measurement of plasma tumor necrosis factor alpha(TNF alpha),interleukin 1 beta(IL-1 beta)and high mobility group protein B1(HMGB-1).Part ?: The effect of different sodium concentration of hypertonic saline resuscitation on the activity of p38 and JNK MAPK in the hepatic tissue of severely scalded rats.The p38 MAPK and JNK activity levels of hepatic tissue in the 5 groups were measured by using Western blotting(Western Blot).Result Part ?: The concentration of ALT and AST in the sham-burn group was significantly lower than the other groups(P <0.01).Compared with LR group,the ALT and AST contents of the 600 HS group decreased significantly(P<0.01)at all times after burn while the 800 HS and 1000 HS group after fluid resuscitation had no significant change at 2,8h after burn(P>0.05),but 800 HS and 1000 HS group decreased significantly(P<0.01)at 24 h after burn.The ALT and AST content of the 800 HS and 1000 HS group is relatively higher than 600 HS at 2,8h post burn(P <0.01),while there were no differences in HS groups at 24 h after burn.The HE staining showed that there were significant fat-sample pathological change and cell edema in the LR group at all times after burn,these pathological changes increaseed with the rising concentration of Na~+ at 2 and 8h after burn.But at 24 h after burn,all the HS groups were the same.Part ?: The concentration of the TNF-?,IL-1? levels of blood in the sham-burn group was significantly lower than the LR group,800 HS group and 1000 HS group(P <0.01),while the 600 HS group had no significant change(P>0.05).Compared with LR group,the TNF-?,IL-1? levels of blood in the 600 HS group decreased significantly(P<0.01)while the 800 HS and 1000 HS group after fluid resuscitation had no significant change(P>0.05)at all times after burn.However,at 2h after burn,the hmgb-1levels of 5groups were the same.At 8h and 24 h after burn,compared with sham-burn group,the hmgb-1levels of LR,800 HS and 1000 HS were significantly higher(P<0.01)except 600 HS group.Compared with LR group,all the HS groups were lower significantly(P<0.01).Compared with 600 HS group,800 and 1000 HS group were higher significantly(P <0.01).The content of liver tissue MDA in shamburn group at each time point were lower than the other groups significantly(P <0.01),while the SOD content of liver tissue in sham-burn group at all times after burn were higher than the other groups significantly(P <0.01).Compared with LR group,the MDA contents of the 600 HS group decreased significantly(P<0.01)but the SOD contents increased significantly(P<0.01)while the 800 HS and 1000 HS group had no significant change(P>0.05)at all times after burn.Compared with 600 HS group,the MDA content of 800 and 1000 HS group were higher significantly(P <0.01),while the SOD content were lower significantly(P <0.01).Part ?: The activity of P38 and JNKMAPK in liver tissues show: the activity expression of hepatic tissue p38 and JNKMAPK in LR group and all HS groups at all times were higher than that of the sham-burn group significantly(P <0.01),the activity expression of liver tissue p38 and JNKMAPK in 600 HS group at each time point was lower than that in LR,800 HS and 1000 HS group significantly(P <0.01),while the activity expression of liver tissue p38 and JNK MAPK in 800 HS and 1000HS group was same(P>0.05).Conclusion(1)Compared with LR group,using 600mmol/L HS for fluid resuscitation can prevent the occurrence of hepatic dysfunction,reduce the damge of liver tisssue and cellular stracture in severely scalded rats.But the effect will be worse with the HS concentration rising to 800 and 1000 mmol /LNa~+.(2)Compared with LR group and other HS groups,using 600mmol/L HS for fluid resuscitation can reduce inflammatory reaction effectively,and reduce liver oxidative damage in severely scalded rats.But the effect will be worse with the HS concentration rising to 800 and 1000 mmol /L Na~+.(3)Compared with LR group and other HS groups,using 600mmol/L HS for fluid resuscitation in severely scalded rats can reduce the activity expression of p38 and JNK MAPK in hepatic tissue.But the effect will be worse with the Na~+ concentration rising to 800 and 1000 mmol /L Na~+.