Experimental Study Of Rabbit On The Limited Fluid Resuscitation Of Uncontrolled Hemorrhagic Shock

Objective：1.To search for the optimal mean arterial pressure (MAP) level during the fluidresuscitation for an uncontrolled shock.2.Maintaining this ideal blood pressure levels,How long it will not seriously affectorgan function..Methods: Based on Wigger's describtion, the animal models with uncontrolledhemorrhagic shock were established by femoral artery phlebotomy. Thirty rabbits wererandomly divided into five groups (each group, n=6):(1)Normal control group (NCgroup): intubation an other operations, no bleeding, no recovery;(2) Non-recoverygroup(NR group)control group; N50group:maintaining MAP at50mmHg; N60group:at60mmHg; N70group:at70mmHg. The arterial blood gas analysis (ABGA),including lactic acid, base excess, arterial oxygen tension (PaO₂), arterial carbondioxide tension (PaCO₂), hydrogen ion exponent (pH value), and et al, was performed atshock before,0,60,120,150,180and240minutes after shock, respectively.Results:1.The amount of continued bleeding with the maintenance of elevated MAP levelincreased;the highest in the N70group.NR group at least. No recovery in the NR group,there is no fluid volume,In other groups,the amount of fluid volume with themaintenance of elevated MAP level increased;the highest in the N70group.NR group atleast. N60group the average survival time of up to248.83±36.54min, followed by theN70group202.00±31.02min, N50group112.67±35.16min, the shortest of NR groupwas58.50±29.50min. Groups, the difference was significant.2. HCT and RBC were reduced with the recovery of blood pressure increases. Each timepoint, the differences were statistically significant significant.3. In addition to the NC group, other groups of lactic acid appeared increased, the N60group rose slower,it has been better than the other groups at the point of60,120,150minafter the shock,there was a significant difference. In addition to the NC group, baseexcess increased negative at60min, N60and N70group was better than the N50and theNR group,120min time point in each group were significantly different, the N70group was better than the other groups in addition to the NC group.4. PO₂, PCO₂, pH value has changed after the shock, with the increase of the recoverytime,PO₂decreased, PCO₂increased, pH value lower.In addition to the NC group,mostslow changes in the N60group, better than the other groups, and180min before eachindexbasically maintained at normal levels; abnormal point in time earlier than the N60group in other groups.5. In addition to the NC group, other groups in shock after SOD was lower, MDAincreased, but the N60group was better than the other groups,120min after the start toappear abnormal, the difference was significant.NR group AST increased at the pointof0min, ALT and Cr, Ur change was not statistically significant.6. In addition to the NC group, other groups platelet count dropped significantlyimmediately after shock, after resuscitation, N60and N70groups rebound and thenlower.The number of PLT N70group is higher than other groups,180min afterrecurrence of rapid decrease, while the N60group has begun to emerge in120mi.N50and NR group PLT levels continue to decline, the difference was significant.Conclusions:1. During a limited fluid resuscitation for the rabbits with unconrntrolled hemorrhagicshock, maintaining MAP at60mmHg may be the ideal level of blood pressure。2. Uncontrolled hemorrhagic shock in rabbits given the limited fluid resuscitation, andto maintain MAP at60mmHg when the time to maintain this level of blood pressuredoes not exceed120min, generally does not cause serious damage to vital organs,the prognosis is relatively good.