The Expression Of The Transcription Factor FOXP3 In Peripheral Blood Mononuclear Cells From Patients With Alopecia Areata

Objective:To investigate the expression of transcription factor FOXP3 in Peripheral blood mononuclear cells(PBMCs) from patients with alopecia areata(AA),and to explore the expression levels of the transcription factor FOXP3 changes in alopecia areata patients and its role in the pathogenesis of alopecia areata. Methods:The protein expression of transcription factor FOXP3 were measured by Western-blotting in PBMCs from the 52 alopecia areata patients and 50 healthy volunteers. Results:The expression of transcription factor FOXP3 in the active alopecia areata patients(0.45±0.34), the stable alopecia areata group(0.83±0.40),the normal contorl group(1.33±0.66).The data was analyzed by one-way ANOVA. Signifieant difference was found in active alopecia areata group,stable alopecia areata group and normal contorl group.(F=20.89,P<0.01).The active group were lower than stable group(P<0.05)and normal contorl group(P<0.01).stable group were lower than normal contorl group(P<0.01). The expression of transcription factor FOXP3 in the local alopecia areata patients(0.75±0.43), the exttensive alopecia areata group(0.41±0.26).The data was analyzed by one-way ANOVA. Signifieant difference was found in local alopecia areata group,exttensive alopecia areata group and normal contorl group.(F=19.77,P<0.01).The exttensive group were lower than local group(P<0.05)and normal contorl group(P<0.01).localgroup were lower than normal contorl group(P<0.01).Inaddition the expression levels of protein were negatively correlated with SALT in patients(r=-0.33,P<0.05). Conclusions:The reduced levels of patients with alopecia areata transcription factor protein may be related to cell immune disorders in Alopecia areata; the expression of the transcription factor FOXP3 protein and severity of alopecia areata showd some relevance, it may play an important role in the pathogenesis of alopecia areata.