Empirical Study About The Effect Of Yuping Feng San On The Structure Of Lung Tissue And Expression Of α1-AT And NE Of Passive Moking Rats

Objective: Through making the passive smoking model, to make the lung pathological section, measure the lung index、spleen index、thymus index of the rats and the content of α1-AT and NE in serum, then to observe the variety of lung tissue structure 、 organ index and expression of α1-AT and NE in serum at different time in passive smoking, evaluate the effect of Yuping Feng San in passive smoking rats about the lung tissue structure,organ index and expression of α1-AT and NE.Methods: using 70 SD rats(200 + 20g), half male and half female, using random number table method, they were divided into 7 groups: 7 days, 14 days, 28 days control group, 7 days, 14 days, 28 days of passive smoking group,Drug group. Passive smoking group rats were smoked differently 7 days, 14 days, 28 days, the smoked method is 2 times one day(in the morning and at the afternoon), 9 roots were lighted in 3 times every time, rats in drug group were smoked 30 min before gavaging into the stomach, the dose of Yuping Feng San is 46.3g/Kg·d. Passive smoking group and drug group rats were put to death on the second day after modeling successfully executed, accurately determined the weight of lung, spleen, thymus and then calculated the lung coefficient, the spleen index and thymus index, used HE staining produced the lungs pathological section, and observed changes of pulmonary tissue under light microscope,assayed the content of α1-AT and NE in the serum by using ELISA, the date was processed for statistical analysis.Results: 1. The lung coefficient of different time of passive smoking rats were higher than the same period control group, the difference had statistical significance. Spleen index: 7days, 28 days passive smoking rats were lower than the same period control group, the difference had statistical significance, 14 days has no significance,control groups had no significance, 7days, 28 days passive smoking group compare with 14 days P<0.05,Thymus index: 7days, 28 days passive smoking rats were lower than the same period control group, the difference had statistical significance, 14 days has no significance, different time of control and passive smoking groups had no significance. 2. Different time of passive smoking rats were occurred different degrees of changes in the alveolar, alveolar wall fracture, the infiltration of inflammatory cells and venous hyperemia. 3. The content of α1-AT and NE in different time of passive smoking rats was less than the control group, in addition to NE in 14 days of passive smoking group, other passive smoking group compared with the control group P< 0.05,and α1-AT and NE concentration reached a maximum on the 14 day of passive smoking rats then decreased with passive smoking time. 4. The lung coefficient of model group were significantly higher than the control group and the drug group, and P < 0.05, there was statistical significance, no significant difference in drug group and control group, the spleen index and thymus index of model group rats were lower than in the control group and the drug group, and the P<0.05, there was statistical significance, no significant difference in drug group and control group. 5. The content of α1-AT in model group rats serum were significantly lower than the control group and drug group, and P< 0.05, there was statistical significance, but the control group and the drug group had little difference. 6. The control group rats’ alveolar structure is clear and the difference between the size is not big, no fault in alveolar walls, no congestion in vein, normal bronchial and there was no inflammatory cell infiltration. The alveolar structure of model group rats was not very clear, the size is different, part of alveolar wall breakage and fusion into pulmonary bulla, alveolar wall thickening, inflammatory cell infiltration increased, venous congestion. The drug group rats’ alveolar structure is clear, though the pulmonary bulla were still exist, the alveolar walls were not thick, and the infiltration of inflammatory cells decreased significantly, there was venous congestion situation.Conclusion:Passive smoking can reduce the α1-AT and NE content in serum of rats, and extent is different, the extent damage of lung tissue structure in different time passive smoking were different, passive smoking can rise rats’ lung index, reduce spleen and thymus index. Yuping Feng San enhanced immune function of passive smoking rats by increasing spleen and thymus index, then increased α1-AT and NE content reduce lung index, thus resist against passive smoking, played a certain protective effect.