Expression Of Twist In Colorectal Cancer Liver Metastases And Its Clinical Significance

Objective:Colorectal cancer is one of the common malignant tumors in our country, and its incidence rate is rising. In the developed and urbanized cities, with the increasing pressure from work, the chenging of the structure of diet and lifestyle, the incidence of colorectal cancer increased more obviously. At present, the treatment of colorectal cancer are surgical operation primarily. In order to improve the therapeutic effect, assistant therapies including chemotherapy, radiotherapy, immunotherapy, traditional Chinese medicine therapy and targeted therapy are used to the patients. In recent years, with the deepening of the study of targeted therapy, targeted therapy of colorectal cancer has made some progress, but the treatment still not mature. The key of achieving targeted therapy is finding the right gene which can promote or inhibit the growth of tumor. It is reported that Twist protein may be involved in metastasis of tumor. consequently, in order to provide a new method for targeted therapy or a new molecular indicator for prognosis, we aimed to study the expression of Twist protein in colorectal cancer and liver metastasis tissue and its relationship with the prognosis. Methods:54 cases of Colorectal Cancer Liver Metastases including primary neoplasm, liver metastasis and corresponding normal colorectal mucosa distenced from primary neoplasm more than 10 cm were collected from the tissue bank of center of general surgery of the general hospital of Beijing command of PLA. Expression of Twist were detected in a series of 54 colorectal cancer patients with immunohistochemistry. Results:The expression of Twist was significantly up-regulated in primary neoplasm than liver metastases or normal colorectal mucosa(p<0.001). In primary neoplasm, Twist expression was detected in 30.0%, 64.9% and 85.7% of T2, T3 and T4 stage cases, respectively, and the difference reached to statistical signficance. Kaplan-Meiera and Log-rank survival analysis revealed that the overall survival of Twist-positive primary neoplasm and liver metastases cases was significantly longer than corresponding Twist-negative cases(p=0.034, p=0.031), respectively. However, Cox regression test showed that neither the expression of Twsit in primary neoplasm nor it in liver metastasis was the independent predictive factors for the overall survival of Colorectal Cancer Liver Metastases patients(p=0.548, p=0.742, respectively). Conclusion:The expression of Twist protein was increased in primary neoplasm of colorectal cancer liver metastases, which may be associated with the mechnism of liver metastases. Twist protein may help to predict the overall survival of colorectal cancer liver metastases cases.